一种来自朝鲜刺五加的具有抗炎活性的新皂苷。

A new saponin from Acanthopanax koreanum with anti-inflammatory activity.

作者信息

Dat Le Duc, Thao Nguyen Phuong, Luyen Bui Thi Thuy, Tai Bui Huu, Woo Mi Hee, Manzoor Zahid, Ali Irshad, Koh Young Sang, Kim Young Ho

机构信息

College of Pharmacy, Catholic University of Daegu, Gyeongsan, 38430, Republic of Korea.

College of Pharmacy, Chungnam National University, Daejeon, 34134, Republic of Korea.

出版信息

Arch Pharm Res. 2017 Mar;40(3):311-317. doi: 10.1007/s12272-016-0879-5. Epub 2016 Dec 29.

DOI:10.1007/s12272-016-0879-5

PMID:28035554

Abstract

Twelve saponins were isolated from the leaves of Acanthopanax koreanum, including one new lupane-type triterpene glycoside, named acankoreoside R (1), together with 11 known triterpenoid saponins (2-12). Their structures were elucidated by 1D and 2D nuclear magnetic resonance (NMR), mass spectroscopic data (MS). All of the fractions and isolated saponins were evaluated for anti-inflammatory activities in lipopolysaccharide (LPS)-stimulated bone marrow-derived dendritic cells (BMDCs) by ELISA. Among them, compounds 1-5, 7, 10, and 12 showed strong inhibitions towards interleukin-12 (IL-12) production with IC values ranging from 1.59 to 5.46 µM. Other compounds were weak or inactive toward IL-12 p40 production.

摘要

从辽东楤木叶中分离出12种皂苷，包括一种新的羽扇豆烷型三萜糖苷，命名为刺楤皂苷R（1），以及11种已知的三萜皂苷（2-12）。通过一维和二维核磁共振（NMR）、质谱数据（MS）阐明了它们的结构。通过酶联免疫吸附测定（ELISA）评估了所有组分和分离出的皂苷在脂多糖（LPS）刺激的骨髓来源树突状细胞（BMDCs）中的抗炎活性。其中，化合物1-5、7、10和12对白细胞介素-12（IL-12）的产生表现出强烈抑制作用，IC值范围为1.59至5.46 μM。其他化合物对IL-12 p40的产生作用较弱或无活性。