School of Metallurgy and Environment, Central South University, Changsha, 410083, People's Republic of China.
Institute of Ecology and Rural Environment Planning, Chinese Academy for Environmental Planning, Beijing, People's Republic of China.
Environ Sci Pollut Res Int. 2018 Jan;25(1):132-140. doi: 10.1007/s11356-016-8312-7. Epub 2016 Dec 29.
Polygonum perfoliatum L. is a Mn-tolerant plant as considered having the potential to revegetate in manganese mine wasteland. The glasshouse experiments were carried out to evaluate its tolerance and physiological response in different Mn concentrations (5, 500, 1000, 2000, 5000, and 10,000 μmol L). Absorption bands of P. perfoliatum differed greatly in lipids, proteins, and carbohydrates. With elevated levels of Mn (5-2000 μmol L), absorbance changed little, which demonstrated that lower Mn concentrations had negligible influence on transport functions. As Mn concentrations in excess of 2000 μmol L, absorbance increased slightly but eventually decreased. Furthermore, a hydroponic culture was carried out in order to study its changes of ultrastructure with the increasing Mn concentrations (5, 1000, and 10,000 μmol L). Lower Mn levels with 5 and 1000 μmol L had no breakage function to the ultrastructure of P. perfoliatum. However, as Mn concentration was up to 10,000 μmol L, visible damages began to appear, the quantity of mitochondria in root cells increased, and the granum lamellae of leaf cell chloroplasts presented a disordered state. In comparison with the controls, black agglomerations were found in the cells of P. perfoliatum under the controlling concentration of Mn with 1000 and 10,000 μmol L for 30 days, which became obvious at higher Mn concentrations. As Mn concentration was 10,000 μmol L, a kind of new acicular substance was developed in leaf cells and intercellular spaces, possibly indicating a resistance mechanism in P. perfoliatum. These results confirm that P. perfoliatum shows potential for the revegetation of abandoned manganese tailings.
贯叶连翘是一种 Mn 耐受植物，被认为具有在 Mn 矿废弃地再植被的潜力。本温室实验旨在评估其在不同 Mn 浓度（5、500、1000、2000、5000 和 10000μmol·L-1）下的耐受能力和生理响应。贯叶连翘的吸收带在脂质、蛋白质和碳水化合物方面差异很大。随着 Mn 水平的升高（5-2000μmol·L-1），吸光度变化不大，表明较低的 Mn 浓度对运输功能几乎没有影响。当 Mn 浓度超过 2000μmol·L-1时，吸光度略有增加，但最终下降。此外，还进行了水培培养，以研究其在 Mn 浓度增加（5、1000 和 10000μmol·L-1）时超微结构的变化。较低的 Mn 水平（5 和 1000μmol·L-1）对贯叶连翘的超微结构没有破坏作用。然而，当 Mn 浓度达到 10000μmol·L-1时，可见损伤开始出现，根细胞中线粒体数量增加，叶片细胞叶绿体的基粒片层出现无序状态。与对照相比，在 Mn 浓度为 1000 和 10000μmol·L-1 的控制浓度下，贯叶连翘的细胞中发现了黑色聚集体，在较高的 Mn 浓度下更为明显。当 Mn 浓度为 10000μmol·L-1 时，叶片细胞和细胞间隙中出现了一种新的针状物质，这可能表明贯叶连翘具有一种抗性机制。这些结果证实贯叶连翘具有在废弃的 Mn 尾矿上再植被的潜力。
