Gruhlke Martin C H, Nicco Carole, Batteux Frederic, Slusarenko Alan J
Department of Plant Physiology, RWTH Aachen University, Worringer Weg 1, Aachen 52074, Germany.
Laboratoire d'Immunologie biologique, Hôpital Cochin, Paris 75679, France.
Antioxidants (Basel). 2016 Dec 26;6(1):1. doi: 10.3390/antiox6010001.
Garlic ( L.) has been used as a spice and medicinal plant since ancient times. Garlic produces the thiol-reactive defence substance, allicin, upon wounding. The effects of allicin on human lung epithelium carcinoma (A549), mouse fibroblast (3T3), human umbilical vein endothelial cell (HUVEC), human colon carcinoma (HT29) and human breast cancer (MCF7) cell lines were tested. To estimate toxic effects of allicin, we used a standard MTT-test (methylthiazoltetrazolium) for cell viability and ³H-thymidine incorporation for cell proliferation. The glutathione pool was measured using monobromobimane and the formation of reactive species was identified using 2',7'-dichlorofluoresceine-diacetate. The YO-PRO-1 iodide staining procedure was used to estimate apoptosis. Allicin reduced cell viability and cell proliferation in a concentration dependent manner. In the bimane test, it was observed that cells treated with allicin showed reduced fluorescence, suggesting glutathione oxidation. The cell lines tested differed in sensitivity to allicin in regard to viability, cell proliferation and glutathione oxidation. The 3T3 and MCF-7 cells showed a higher proportion of apoptosis compared to the other cell types. These data show that mammalian cell lines differ in their sensitivity and responses to allicin.
大蒜（L.）自古以来就被用作香料和药用植物。大蒜在受伤时会产生硫醇反应性防御物质大蒜素。测试了大蒜素对人肺上皮癌（A549）、小鼠成纤维细胞（3T3）、人脐静脉内皮细胞（HUVEC）、人结肠癌（HT29）和人乳腺癌（MCF7）细胞系的影响。为了评估大蒜素的毒性作用，我们使用标准的MTT试验（甲基噻唑四唑）检测细胞活力，使用³H-胸腺嘧啶核苷掺入法检测细胞增殖。使用单溴代双马来酰胺测量谷胱甘肽池，并使用2',7'-二氯荧光素二乙酸酯鉴定活性物质的形成。采用YO-PRO-1碘化物染色法评估细胞凋亡。大蒜素以浓度依赖的方式降低细胞活力和细胞增殖。在双马来酰胺试验中，观察到用大蒜素处理的细胞荧光降低，表明谷胱甘肽氧化。所测试的细胞系在活力、细胞增殖和谷胱甘肽氧化方面对大蒜素的敏感性不同。与其他细胞类型相比，3T3和MCF-7细胞显示出更高比例的细胞凋亡。这些数据表明，哺乳动物细胞系对大蒜素的敏感性和反应不同。
