食管鳞状细胞癌活检组织的细胞在重组基底膜中的生长

Growth of cells from esophageal squamous cell carcinoma biopsies in a reconstituted basement membrane.

作者信息

Percario M, Melchiori A, Aresu O, Ferreri-Santi L, Carlone S, Parodi S, Conio M, Munizzi F, Aste H, Albini A

出版信息

Boll Soc Ital Biol Sper. 1989 Aug;65(8):775-81.

Abstract

Cells from esophageal carcinoma biopsies were cultured on or inside a three-dimensional basement membrane matrix (matrigel). Their growth was compared to cells derived from control esophageal biopsies. Cells from both normal and neoplastic tissue attached poorly to tissue culture plastic. Matrigel coating improved adhesion and growth. When cells were grown inside a matrigel matrix, a striking difference was noticed between carcinoma cells and controls. Carcinoma cells grew invasively in the three-dimensional substrate and digested the matrix after a few weeks; control cells did not grow and only a few necrotic cells were visible with time. Matrigel provided a better growth substrate than plastic for esophageal derived cells and discriminated between carcinoma-derived and control cells when used as a three-dimensional growth substrate. Our studies suggest a possible use of matrigel for the selective growth of tumor cells derived directly from tissue biopsies.

摘要

取自食管癌活检组织的细胞在三维基底膜基质（基质胶）上或其内部进行培养。将它们的生长情况与取自对照食管活检组织的细胞进行比较。来自正常组织和肿瘤组织的细胞在组织培养塑料上的附着性都很差。基质胶包被可改善细胞黏附及生长情况。当细胞在基质胶基质内部生长时，癌细胞与对照细胞之间出现了显著差异。癌细胞在三维基质中呈侵袭性生长，并在数周后消化基质；对照细胞不生长，随着时间推移仅可见少数坏死细胞。对于源自食管的细胞，基质胶比塑料提供了更好的生长基质，并且当用作三维生长基质时，可区分癌细胞和对照细胞。我们的研究表明，基质胶可能可用于直接源自组织活检的肿瘤细胞的选择性生长。