The Key Lab of Pollution Control and Ecosystem Restoration in Industry Clusters, Ministry of Education, School of Environment and Energy, South China University of Technology, Guangzhou 510006, PR China.
The Key Lab of Pollution Control and Ecosystem Restoration in Industry Clusters, Ministry of Education, School of Environment and Energy, South China University of Technology, Guangzhou 510006, PR China; Guangdong Provincial Engineering and Technology Research Center for Environmental Risk Prevention and Emergency Disposal, South China University of Technology, Guangzhou 510006, PR China.
Sci Total Environ. 2017 Feb 15;580:1371-1380. doi: 10.1016/j.scitotenv.2016.12.102. Epub 2016 Dec 27.
The necessity for developing an efficient and cost-effective in situ bioremediation technology for sediments contaminated with polychlorinated biphenyls (PCBs) has prompted the application of low-voltage electrical fields to anaerobic digestion systems. Here we show that the use of a sediment-based bio-electrochemical reactor (BER) poised at a potential of -0.50V (vs. a standard calomel electrode, SCE) substantially enhanced the reduction of 2,3,4,5-tetrachlorobiphenyl (PCB 61) when acetate was added as a carbon source. The addition of surfactant Tween 80 to the BER further accelerated the PCB 61 transformation. The comparative study of closed- and open-circuit reactors demonstrated the enrichment conditions affecting the bacterial community structure, the dominant dechlorination metabolisms, and thus the extent, the rate and the products of the reduction of PCBs. The dominant bacterial dechlorinators detected in the BERs in the presence of acetate and Tween 80 are Dehalogenimonas, Dehalobacter, Sulfuricurvum, Dechloromonas and Geobacter, which should be responsible for PCB dechlorination. This study improves understanding of the key factors influencing dechlorination activity in sediment-based BERs polarized at a low potential, as well as the metabolic mechanisms dominating in the PCB dechlorination process.
为了开发一种高效且经济实用的原位生物修复技术来处理被多氯联苯（PCBs）污染的沉积物，人们已经将低电压电场应用于厌氧消化系统。在这里，我们发现，当向基于沉积物的生物电化学反应器（BER）中添加乙酸盐作为碳源，并将其设定在-0.50V（相对于标准甘汞电极，SCE）的电势时，可以显著增强 2,3,4,5-四氯联苯（PCB 61）的还原。向 BER 中添加表面活性剂吐温 80 进一步加速了 PCB 61 的转化。对闭路和开路反应器的比较研究表明，富集条件会影响细菌群落结构、主要的脱氯代谢途径，从而影响 PCB 还原的程度、速率和产物。在含有乙酸盐和吐温 80 的 BER 中检测到的主要脱氯菌是 Dehalogenimonas、Dehalobacter、Sulfuricurvum、Dechloromonas 和 Geobacter，它们应该负责 PCB 的脱氯。这项研究提高了对影响低电位极化的基于沉积物的 BER 中脱氯活性的关键因素以及在 PCB 脱氯过程中起主导作用的代谢机制的理解。
