Graduate Program in Pharmaceutical Sciences, Federal University of Santa Maria, Avenida Roraima 1000, Camobi Campus, Santa Maria, RS 97105-900, Brazil.
Graduate Program in Biological Sciences: Toxicological Biochemistry, Federal University of Santa Maria, Camobi Campus, Santa Maria, RS 97105-900, Brazil.
J Photochem Photobiol B. 2017 Feb;167:29-35. doi: 10.1016/j.jphotobiol.2016.12.026. Epub 2016 Dec 22.
Scutia buxifolia Reissek (Rhamnaceae), popularly known in Brazil as "coronilha", is a plant species used in folk medicine for several disorders, including inflammation. However, no studies have been done with this species to confirm its topical anti-inflammatory action. In this study we evaluate the topical antiedematogenic and anti-inflammatory effects of the gel containing crude extract (CE) and the gel containing ethyl acetate (EtOAc) fraction of S. buxifolia on croton oil or UVB radiation-induced ear edema in mice, and perform gel stability study. Antiedematogenic and anti-inflammatory effects were evaluated through ear edema induced by irritant agent croton oil, UVB irradiation-induced skin injury model and neutrophil infiltration. The gel stability study was performed by analyzing organoleptical aspects, pH, viscosity, and quantification of quercetin and rutin by HPLC. The topical treatment with S. buxifolia gel reduced the ear edema and myeloperoxidase activity. Antiedematogenic and anti-inflammatory effects of S. buxifolia were obtained with concentrations of 0.3, 1 and 3%, with maximal inhibition in the concentration of 1% for gel containing CE (inhibitions of 100, 73±0.05 and 97±0.08% for croton oil- or UVB irradiation-induced ear edema and myeloperoxidase activity, respectively) and EtOAc fraction (inhibitions of 79±0.05, 73±0.05 and 89±0.04% for croton oil- or UVB irradiation-induced ear edema and myeloperoxidase activity, respectively). Such effects may be attributed, at least in part, to rutin and quercetin, as well as other compounds found in this species. No important changes were detected in the stability study, in all aspects analyzed in temperature below 25°C. Our results demonstrate that topically applied S. buxifolia gel presented anti-inflammatory effects, provided that it was maintained at a temperature below 25°C.
金腰箭（茜草科），俗称巴西的“coronilha”，是一种在民间医学中用于多种疾病的植物物种，包括炎症。然而，尚未对该物种进行研究以确认其局部抗炎作用。在这项研究中，我们评估了含有金腰箭粗提取物（CE）的凝胶和含有乙酸乙酯（EtOAc）部分的凝胶对小鼠巴豆油或 UVB 辐射诱导的耳水肿的局部抗肿胀和抗炎作用，并进行了凝胶稳定性研究。通过致炎剂巴豆油诱导的耳水肿、UVB 辐射诱导的皮肤损伤模型和中性粒细胞浸润来评估抗肿胀和抗炎作用。通过分析光物理方面、pH 值、粘度以及通过 HPLC 定量槲皮素和芦丁来进行凝胶稳定性研究。金腰箭凝胶的局部治疗可减轻耳水肿和髓过氧化物酶活性。金腰箭的抗肿胀和抗炎作用是通过浓度为 0.3%、1%和 3%获得的，CE 凝胶的浓度为 1%时最大抑制作用（对巴豆油或 UVB 辐射诱导的耳水肿和髓过氧化物酶活性的抑制率分别为 100%、73±0.05%和 97±0.08%）和 EtOAc 部分（对巴豆油或 UVB 辐射诱导的耳水肿和髓过氧化物酶活性的抑制率分别为 79±0.05%、73±0.05%和 89±0.04%）。这些作用可能至少部分归因于芦丁和槲皮素以及该物种中发现的其他化合物。在低于 25°C 的所有分析方面的稳定性研究中，未检测到重要变化。我们的结果表明，金腰箭凝胶的局部应用具有抗炎作用，只要将其保持在低于 25°C 的温度下。
