NEIKER, Department of Conservation of Natural Resources, Soil Microbial Ecology Group, c/ Berreaga 1, E-48160 Derio, Spain.
NEIKER, Department of Conservation of Natural Resources, Soil Microbial Ecology Group, c/ Berreaga 1, E-48160 Derio, Spain.
Sci Total Environ. 2017 Apr 15;584-585:329-338. doi: 10.1016/j.scitotenv.2016.12.146. Epub 2016 Dec 29.
Mining sites shelter a characteristic biodiversity with large potential for the phytoremediation of metal contaminated soils. Endophytic plant growth-promoting bacteria were isolated from two metal-(hyper)accumulator plant species growing in a metal contaminated mine soil. After characterizing their plant growth-promoting traits, consortia of putative endophytes were used to carry out an endophyte-assisted phytoextraction experiment using Noccaea caerulescens and Rumex acetosa (singly and in combination) under controlled conditions. We evaluated the influence of endophyte-inoculated plants on soil physicochemical and microbial properties, as well as plant physiological parameters and metal concentrations. Data interpretation through the grouping of soil properties within a set of ecosystem services was also carried out. When grown together, we observed a 41 and 16% increase in the growth of N. caerulescens and R. acetosa plants, respectively, as well as higher values of Zn phytoextraction and soil microbial biomass and functional diversity. Inoculation of the consortia of putative endophytes did not lead to higher values of plant metal uptake, but it improved the plants' physiological status, by increasing the content of chlorophylls and carotenoids by up to 28 and 36%, respectively, indicating a reduction in the stress level of plants. Endophyte-inoculation also stimulated soil microbial communities: higher values of acid phosphatase activity (related to the phosphate solubilising traits of the endophytes), bacterial and fungal abundance, and structural diversity. The positive effects of plant growth and endophyte inoculation on soil properties were reflected in an enhancement of some ecosystem services (biodiversity, nutrient cycling, water flow regulation, water purification and contamination control).
矿区蕴藏着具有很大潜力的特征生物多样性，可用于修复受金属污染的土壤。从生长在受金属污染矿区土壤中的两种金属（超）积累植物物种中分离出了植物内生促生细菌。在对其促进植物生长的特性进行表征后，使用推定的内生菌共生体进行了内生菌辅助植物提取实验，实验使用了 Noccaea caerulescens 和 Rumex acetosa（单独和组合），并在受控条件下进行。我们评估了内生菌接种植物对土壤物理化学和微生物特性以及植物生理参数和金属浓度的影响。还通过将土壤特性分组为一组生态系统服务来解释数据。当一起生长时，我们观察到 N. caerulescens 和 R. acetosa 植物的生长分别增加了 41%和 16%，以及更高的 Zn 植物提取值和土壤微生物生物量和功能多样性。共生体的接种并没有导致植物对金属吸收的更高值，但它通过将叶绿素和类胡萝卜素的含量分别提高 28%和 36%，改善了植物的生理状态，表明植物的压力水平降低。内生菌接种还刺激了土壤微生物群落：酸性磷酸酶活性（与内生菌的解磷特性有关）、细菌和真菌丰度以及结构多样性的更高值。植物生长和内生菌接种对土壤特性的积极影响反映在一些生态系统服务（生物多样性、养分循环、水流调节、水净化和污染控制）的增强上。
