负载阿霉素和超顺磁性氧化铁的微多糖药物载体用于肝癌双模态治疗的研发与应用
Development and application of micro-polysaccharide drug carriers incorporating doxorubicin and superparamagnetic iron oxide for bimodality treatment of hepatocellular carcinoma.
作者信息
Tang Sung-Ling, Bai Meng-Yi, Wang Jyun-Yi, Hong Po-Da
机构信息
Biomedical Engineering Program, Graduate Institute of Applied Science and Technology, National Taiwan University of Science and Technology, Taipei, Taiwan, ROC.
Biomedical Engineering Program, Graduate Institute of Applied Science and Technology, National Taiwan University of Science and Technology, Taipei, Taiwan, ROC; Graduate Institute of Biomedical Engineering, National Taiwan University of Science and Technology, Taipei, Taiwan, ROC; Adjunct Appointment to the Department of Biomedical Engineering, National Defense Medical Center, Taipei, Taiwan, ROC.
出版信息
Colloids Surf B Biointerfaces. 2017 Mar 1;151:304-313. doi: 10.1016/j.colsurfb.2016.12.036. Epub 2016 Dec 23.
In this study, we demonstrated a novel polyelectrolyte microparticle, doxorubicin(DOX)-superparamagnetic iron oxide (SPIO)-chondroitin sulfate (CS)/chitosan (CHI)microparticles (MPs), as a drug delivery system for hepatic cancer treatment. We also investigated the properties of these microparticles through composition determination, formulation tests, in vitro study, and in vivo study. The results showed that our DOX-SPIO-CS/CHI MPs had an average diameter of 1.43±0.54μm and exhibited a spherical shape. The encapsulation efficiency of this drug carrier was approximately 31±8.07%, according to our spectroscopic determination. The results of release profile test revealed the sustained-release behavior of DOX-SPIO-CS/CHI MPs, which released 51.5% of DOX within 48h of the testing period. According to the results of a cell viability assay and an animal study, the DOX-SPIO-CS/CHI MPs exhibited stronger cytotoxicity than did free DOX when it was administered to Hep G2 and Huh-6 human liver cancer cell lines in vitro and to nude mice of Hep G2/Huh-6-bearing mice model in vivo.
在本研究中，我们展示了一种新型聚电解质微粒，即阿霉素（DOX）-超顺磁性氧化铁（SPIO）-硫酸软骨素（CS）/壳聚糖（CHI）微粒（MPs），作为一种用于肝癌治疗的药物递送系统。我们还通过成分测定、制剂测试、体外研究和体内研究来研究这些微粒的性质。结果表明，我们的DOX-SPIO-CS/CHI MPs平均直径为1.43±0.54μm，呈球形。根据我们的光谱测定，这种药物载体的包封率约为31±8.07%。释放曲线测试结果显示了DOX-SPIO-CS/CHI MPs的缓释行为，在测试期48小时内释放了51.5%的DOX。根据细胞活力测定和动物研究结果，当将DOX-SPIO-CS/CHI MPs体外应用于Hep G2和Huh-6人肝癌细胞系以及体内应用于荷Hep G2/Huh-6裸鼠模型时，其表现出比游离DOX更强的细胞毒性。
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