Fein A, Vechoropoulos M, Nebel L
Department of Embryology and Teratology, Tel-Aviv University Medical School, Tel-Hashomer, Israel.
Biol Neonate. 1989;56(3):165-73. doi: 10.1159/000243118.
Pregnant ICR mice were injected daily with 30 mg cyclosporin (CS)/kg body weight on days 6-8 or 10-12 of gestation. In parallel, control mice were administered saline injections on the same gestational days. The mice were sacrificed on days 12, 15, 17 or 19 of gestation. The number of resorption sites counted, the embryos and placentae were carefully separated, weighed and examined macro- and microscopically, along with various other maternal organs. It was found that 30 mg CS/kg body weight, when administered to pregnant ICR mice in 3 successive injections, did not raise the maternal mortality rate. Histological examination of maternal organs revealed pathological alterations in the thymus, liver, kidney and spleen; most changes had disappeared by 1 week following the last injection. CS reduced the number of viable embryos and increased the number of embryos resorbed. Microscopic examination of the embryos showed that organogenesis was not affected by the drug. However, CS, in the administered dose, had a clear embryotoxic effect.
妊娠第6至8天或第10至12天，每天给怀孕的ICR小鼠注射30毫克环孢素（CS）/千克体重。同时，在相同的妊娠天数给对照小鼠注射生理盐水。在妊娠第12、15、17或19天处死小鼠。计算吸收位点的数量，小心分离胚胎和胎盘，称重并进行大体和显微镜检查，同时检查各种其他母体器官。结果发现，连续3次给怀孕的ICR小鼠注射30毫克CS/千克体重，并未提高母体死亡率。对母体器官的组织学检查显示，胸腺、肝脏、肾脏和脾脏出现了病理改变；大多数变化在最后一次注射后1周内消失。CS减少了存活胚胎的数量，增加了吸收胚胎的数量。对胚胎的显微镜检查表明，器官发生不受该药物影响。然而，所给剂量的CS具有明显的胚胎毒性作用。
