小鼠造血干细胞全转录组剪接图谱分析

Mapping Whole-Transcriptome Splicing in Mouse Hematopoietic Stem Cells.

作者信息

Goldstein Oron, Meyer Karin, Greenshpan Yariv, Bujanover Nir, Feigin Mili, Ner-Gaon Hadas, Shay Tal, Gazit Roi

机构信息

The Shraga Segal Department for Microbiology, Immunology and Genetics, Faculty of Health Science, Ben-Gurion University of the Negev, Be'er Sheva 84105, Israel; The National Institute for Biotechnology in the Negev, Ben-Gurion University of the Negev, Be'er Sheva 84105, Israel.

The Shraga Segal Department for Microbiology, Immunology and Genetics, Faculty of Health Science, Ben-Gurion University of the Negev, Be'er Sheva 84105, Israel.

出版信息

Stem Cell Reports. 2017 Jan 10;8(1):163-176. doi: 10.1016/j.stemcr.2016.12.002. Epub 2016 Dec 29.

Hematopoietic stem cells (HSCs) are rare cells that generate all the various types of blood and immune cells. High-quality transcriptome data have enabled the identification of significant genes for HSCs. However, most genes are expressed in various forms by alternative splicing (AS), extending transcriptome complexity. Here, we delineate AS to determine which isoforms are expressed in mouse HSCs. Our analysis of microarray and RNA-sequencing data includes differential expression of splicing factors that may regulate AS, and a complete map of splicing isoforms. Multiple types of isoforms for known HSC genes and unannotated splicing that may alter gene function are presented. Transcriptome-wide identification of genes and their respective isoforms in mouse HSCs will open another dimension for adult stem cells.

造血干细胞（HSCs）是一种罕见的细胞，可生成所有不同类型的血液和免疫细胞。高质量的转录组数据有助于识别造血干细胞的重要基因。然而，大多数基因通过可变剪接（AS）以多种形式表达，从而增加了转录组的复杂性。在这里，我们通过可变剪接来确定哪些异构体在小鼠造血干细胞中表达。我们对微阵列和RNA测序数据的分析包括可能调节可变剪接的剪接因子的差异表达，以及剪接异构体的完整图谱。文中展示了已知造血干细胞基因的多种异构体类型以及可能改变基因功能的未注释剪接。对小鼠造血干细胞中基因及其各自异构体进行全转录组范围的鉴定将为成体干细胞研究开辟新的维度。