Ye Mao, Song Yuan-Yuan, Baerson Scott R, Long Jun, Wang Jie, Pan Zhiqiang, Lin Wen-Xiong, Zeng Ren-Sen
State Key Laboratory of Conservation and Utilization of Subtropical Agro-bioresources, College of Natural Resources and Environment, South China Agricultural University, Wushan, Guangzhou, China.
Key Laboratory of Ministry of Education for Genetics, Breeding and Multiple Utilization of Crops, College of Crop Science, Fujian Agriculture and Forestry University, Jinshan, Fuzhou, 350002, China.
Plant Cell Environ. 2017 May;40(5):779-787. doi: 10.1111/pce.12897. Epub 2017 Feb 23.
Rice ratooning is practiced in many rice-growing countries for achieving increased rice production with limited labour input. Here, we report that attack by insect herbivores, or treatment with a defense signaling compound in parent plants, can prime anti-herbivore defense responses in subsequent ratoon plants. We compared the defense responses of rice ratoons generated from parent plants that had been either infested by Cnaphalocrocis medinalis (rice leaffolder, LF) caterpillars or treated with methyl jasmonate (MeJA) during vegetative growth, with ratoons generated from control parent plants. Ratoon plants generated from parents receiving prior LF infestation or MeJA treatment exhibited higher jasmonic acid (JA) levels, as well as elevated levels of transcripts of defense-related genes associated with JA signaling. In addition, elevated activities of peroxidase, polyphenol oxidase and trypsin protease inhibitor were observed, as well as enhanced resistance towards subsequent LF infestation. Pre-priming of ratoon defense responses was significantly reduced in plants where expression of OsAOS (allene oxide synthase, involved in JA biosynthesis) or OsCOI1 (CORONATINE INSENSITIVE1, involved in JA perception) was inhibited by RNA interference. Our results indicate that herbivore exposure or MeJA treatment in rice parent plants enhances anti-herbivore resistance in subsequently generated ratoons through priming of JA-mediated defenses.
许多水稻种植国家都采用再生稻种植方式，以便在劳动力投入有限的情况下提高水稻产量。在此，我们报告称，食草昆虫的侵害或母本植物用防御信号化合物处理，可使后续再生植株产生抗食草动物防御反应。我们比较了在营养生长阶段遭受稻纵卷叶螟（LF）幼虫侵害或用茉莉酸甲酯（MeJA）处理的母本植物所产生的再生稻，与对照母本植物所产生的再生稻的防御反应。来自先前遭受LF侵害或接受MeJA处理的母本的再生植株，茉莉酸（JA）水平较高，与JA信号传导相关的防御相关基因转录水平也有所升高。此外，还观察到过氧化物酶、多酚氧化酶和胰蛋白酶抑制剂的活性增强，以及对后续LF侵害的抗性增强。在通过RNA干扰抑制了OsAOS（丙二烯氧化物合酶，参与JA生物合成）或OsCOI1（冠菌素不敏感1，参与JA感知）表达的植株中，再生稻防御反应的预激发显著降低。我们的结果表明，水稻母本植物遭受食草动物侵害或接受MeJA处理，可通过激发JA介导的防御，增强后续再生稻的抗食草动物抗性。
