Wu S L, Xu L R, Zhou Z J
Department of Medical Technology, Xiamen Medical University, Xiamen 361008, China.
Zhonghua Lao Dong Wei Sheng Zhi Ye Bing Za Zhi. 2016 Nov 20;34(11):801-804. doi: 10.3760/cma.j.issn.1001-9391.2016.11.001.
To explore effect of fluorochloridone on primary co-cultured sertoli-germ cell of rat and its possible mechanism. primary co-cultured sertoli-germ cell was made by two steps of en-zyme digestion with SD rat testes, after 24h of Sertoli-germ cell isolation, A 0.1% DMSO solvent control group and three FLC exposure groups (10、10、10 mol/L) were selected, cultured cell for 24h, then MTT assay and index detection of oxidative stress were performed. The mortality of primary co-cultured sertoli-germ cell exposed to 10 mol/L FLC was significantly higher than control group and FLC exposure groups (10、10 mol/L) (<0.05) . 10 mol/L FLC reduced enzyme activity of CAT, SOD and GSH. Px, depressed GSH level, el-evated MDA level, and had significant difference than control group and FLC exposure groups(10、10 mol/L) (<0.05) . 10 mol/L FLC decreased enzyme activity of CAT than control group, depressed enzyme activity of SOD than 10 mol/L FLC, lowered GSH level than control group and FLC exposure groups (10 mol/L) , and had statistical difference (<0.05) . FLC can damage primary co-cultured sertoli-germ cell of rat, its possible mechanism is relevant to oxidative stress.
