Ziecik A J, Klos J, Przygrodzka E, Milewski R, Jana B
Institute of Animal Reproduction and Food Research, Polish Academy of Sciences, Olsztyn, Poland.
Institute of Animal Reproduction and Food Research, Polish Academy of Sciences, Olsztyn, Poland.
Theriogenology. 2017 Feb;89:250-254. doi: 10.1016/j.theriogenology.2016.10.028. Epub 2016 Nov 9.
Research was conducted to determine the effect of altrenogest and exposure to exogenous gonadotropins on ovarian function in prepubertal and mature gilts. Crossbred, presumably sexually mature gilts (n = 51), were fed with altrenogest for 18 consecutive days and the day after the last feeding with altrenogest, gilts were treated with eCG and 72 hours later challenged with hCG. Animals were slaughtered on Days 10 to 13 of their gonadotropins synchronized estrous cycle. Ovaries were examined for the number of CL, number of follicular cysts, and presence of corpora albicantia. Gilts were divided into two groups: those possessing corpora albicantia (group A-mature; n = 36) and those without corpora albicantia (Group W-prepubertal; n = 15) on their ovaries. In addition, each group was divided into two subgroups depending on the presence of follicular cysts (AC and WC) or their absence (AO and WO). There was no difference between the number of CL in group A and group W. Presence of corpora albicantia determined percentage of gilts possessing follicular cysts (13.9% group A vs. 66.7% group W). Gilts without follicular cysts (AO plus WO; n = 36) had higher number of CL (P < 0.01) than gilts bearing cysts (AC plus WC; n = 15). Comparison AO-AC did not show significant difference (P = 0.075) between CL number in mature cyst-free and cysts bearing gilts. A prepubertal gilts not bearing follicular cysts (WO) had higher (P < 0.02) number of CL than gilts bearing cysts. A significant negative correlation between the number of CL and number of follicular cysts was found (r = -0.664; P = 0.007). There were no differences in blood plasma progesterone and estradiol concentration between cyst-free and cyst-bearing gilts. These results indicate: (1) a higher follicular cysts appearance in prepubertal than mature gilts challenged with altrenogest and exposed to exogenous gonadotropins and (2) a negative effect of follicular cysts on the number of CL (ovulations) in prepubertal gilts.
开展了一项研究，以确定烯丙孕素和接触外源性促性腺激素对青春期前和成熟后备母猪卵巢功能的影响。选用51头杂种、推测性成熟的后备母猪，连续18天饲喂烯丙孕素，在最后一次饲喂烯丙孕素后的第二天，给母猪注射孕马血清促性腺激素（eCG），72小时后再注射人绒毛膜促性腺激素（hCG）。在其促性腺激素同步发情周期的第10至13天屠宰动物。检查卵巢上的黄体（CL）数量、卵泡囊肿数量以及白体的存在情况。将母猪分为两组：卵巢上有白体的（A组 - 成熟；n = 36）和卵巢上没有白体的（W组 - 青春期前；n = 15）。此外，根据卵泡囊肿的有无，每组又分为两个亚组（AC和WC）或无囊肿组（AO和WO）。A组和W组的黄体数量没有差异。白体的存在决定了有卵泡囊肿的母猪百分比（A组为13.9%，W组为66.7%）。无卵泡囊肿的母猪（AO加WO；n = 36）的黄体数量（P < 0.01）高于有囊肿的母猪（AC加WC；n = 15）。AO - AC比较显示，成熟无囊肿和有囊肿母猪的黄体数量之间没有显著差异（P = 0.075）。青春期前无卵泡囊肿的母猪（WO）的黄体数量比有囊肿的母猪更高（P < 0.02）。发现黄体数量与卵泡囊肿数量之间存在显著负相关（r = -0.664；P = 0.007）。无囊肿和有囊肿母猪的血浆孕酮和雌二醇浓度没有差异。这些结果表明：（1）在接受烯丙孕素和接触外源性促性腺激素刺激的情况下，青春期前母猪比成熟母猪出现卵泡囊肿的几率更高；（2）卵泡囊肿对青春期前母猪的黄体数量（排卵数）有负面影响。
