巴西首次报道马驹感染人隐孢子虫GP60基因型IkA20G1以及微小隐孢子虫GP60基因型IIaA18G3R1和IIaA15G2R1 。
First description of Cryptosporidium hominis GP60 genotype IkA20G1 and Cryptosporidium parvum GP60 genotypes IIaA18G3R1 and IIaA15G2R1 in foals in Brazil.
作者信息
Inácio Sandra Valéria, Widmer Giovanni, de Brito Roberta Lomonte Lemos, Zucatto Anaiza Simão, de Aquino Monally Conceição Costa, Oliveira Bruno César Miranda, Nakamura Alex Akira, Neto Luiz da Silveira, Carvalho João Gabriel Balizardo, Gomes Jancarlo Ferreira, Meireles Marcelo Vasconcelos, Bresciani Katia Denise Saraiva
机构信息
Universidade Estadual Paulista (UNESP), Faculdade de Medicina Veterinária, Araçatuba, Departamento de Apoio, Produção e Saúde Animal, Araçatuba, São Paulo, Brazil.
Department of Infectious Disease & Global Health, Cummings School of Veterinary Medicine at Tufts University, North Grafton, MA, USA.
出版信息
Vet Parasitol. 2017 Jan 15;233:48-51. doi: 10.1016/j.vetpar.2016.11.021. Epub 2016 Dec 2.
The present study focuses on Cryptosporidium infections of foals in Brazil. A total of 92 animals of different breeds from 11 farms in the vicinity of Araçatuba in the state of São Paulo, were examined. According to PCR targeting the 18S rRNA gene, Cryptosporidium sp. DNA was detected in 21.7% (20/92) of foals. Good quality 18S rRNA, actin, HSP70 and gp60 genes nPCR amplicons were obtained from five fecal samples. PCR amplification and sequencing of a fragment of the GP60 sporozoite surface glycoprotein gene revealed C. parvum genotypes IIaA18G3R1, IIaA15G2R1. Interestingly, we also detected in two foals a GP60 genotype related to the human parasite C. hominis.
本研究聚焦于巴西马驹的隐孢子虫感染情况。对圣保罗州阿拉萨图巴附近11个农场的92匹不同品种的动物进行了检查。根据针对18S rRNA基因的PCR检测，在21.7%（20/92）的马驹中检测到隐孢子虫属DNA。从5份粪便样本中获得了高质量的18S rRNA、肌动蛋白、HSP70和gp60基因nPCR扩增子。对GP60子孢子表面糖蛋白基因片段进行PCR扩增和测序，发现了微小隐孢子虫基因型IIaA18G3R1、IIaA15G2R1。有趣的是，我们还在两匹马驹中检测到了与人类寄生虫人隐孢子虫相关的GP60基因型。
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