在浓乙腈蒸气中进行电喷雾电离可提高蛋白质组学分析的质谱性能。

Electrospray ionization in concentrated acetonitrile vapor improves the performance of mass spectrometry for proteomic analyses.

作者信息

Chen Jin, Wang Fangjun, Liu Zheyi, Liu Jing, Zhu Yixin, Zhang Yukui, Zou Hanfa

机构信息

CAS Key Laboratory of Separation Sciences for Analytical Chemistry, Dalian Institute of Chemical Physics, Chinese Academy of Sciences (CAS), Dalian 116023, Liaoning, PR China; University of Chinese Academy of Sciences, Beijing 100049, China.

CAS Key Laboratory of Separation Sciences for Analytical Chemistry, Dalian Institute of Chemical Physics, Chinese Academy of Sciences (CAS), Dalian 116023, Liaoning, PR China.

出版信息

J Chromatogr A. 2017 Feb 3;1483:101-109. doi: 10.1016/j.chroma.2016.12.075. Epub 2016 Dec 26.

DOI:10.1016/j.chroma.2016.12.075

PMID:28043689

Abstract

Suppressing the background interferences and enhancing the analytes signals are long-term goals in high performance electrospray ionization mass spectrometry (ESI-MS) analyses. We observed that performing electrospray in the presence of a concentrated acetonitrile atmosphere suppresses background interferences and enhances peptide signals. An enclosed nanoESI source was utilized to provide a stable atmosphere of concentrated acetonitrile vapor for high performance ESI-MS analyses. The median MS signal intensity increased by 5 times for a set of 23 BSA tryptic peptides in direct ESI-MS analysis. Further, the number of reproducibly and precisely quantified peptides could be improved 67% in six replicate label-free quantitative proteome analyses by this strategy.

摘要

抑制背景干扰并增强分析物信号是高效电喷雾电离质谱（ESI-MS）分析中的长期目标。我们观察到，在浓乙腈气氛中进行电喷雾可抑制背景干扰并增强肽信号。利用封闭的纳米电喷雾源为高效ESI-MS分析提供稳定的浓乙腈蒸气气氛。在直接ESI-MS分析中，一组23种牛血清白蛋白胰蛋白酶肽的质谱信号强度中位数增加了5倍。此外，通过该策略，在六次重复的无标记定量蛋白质组分析中，可重复且精确定量的肽数量可提高67%。