von Hof Jessica, Sprekeler Nele, Schuler Gerhard, Boos Alois, Kowalewski Mariusz P
Institute of Veterinary Anatomy, Vetsuisse Faculty, University of Zurich, Winterthurerstrasse 260, 8057 Zurich, Switzerland.
Clinic for Obstetrics, Gynecology and Andrology of Large and Small Animals, Faculty of Veterinary Medicine, Justus-Liebig-University Giessen, 35392 Giessen, Germany.
Prostaglandins Other Lipid Mediat. 2017 Jan;128-129:17-26. doi: 10.1016/j.prostaglandins.2016.12.003. Epub 2016 Dec 30.
15-Hydroxyprostaglandin dehydrogenase (HPGD) plays a key role in prostaglandins (PGs) catabolism. Its expression and activity appear to be regulated by progesterone (P4). We investigated the HPGD mRNA-expression and protein localization in placentomes and interplacental uterine sites throughout gestation (Study I), and after fetal membranes retention (RFM) compared with normally delivered fetal membranes (DFM) (Study II). Furthermore, we analyzed the influence of aglepristone (AP), dexamethasone (GC) or cloprostenol (CP), on HPGD expression in bovine placentomes (Study III). Tissues from late gestation (D272) and at normal term (NT) served as controls. HPGD was highest in all sites at the beginning of pregnancy and at (NT). Following induced parturition HPGD was lower after (AP) and (GC) compared with (NT), and was similar in RFM and DFM. Placentomes stained primarily in fetal compartments; interplacentomal signals were observed in endometrial glandular and luminal epithelium. Results indicate that HPGD may play a role during establishment and termination of gestation.
15-羟基前列腺素脱氢酶（HPGD）在前列腺素（PGs）分解代谢中起关键作用。其表达和活性似乎受孕酮（P4）调节。我们研究了整个妊娠期胎盘小叶和胎盘间子宫部位的HPGD mRNA表达和蛋白定位（研究I），并将胎膜残留（RFM）后的情况与正常分娩的胎膜（DFM）进行比较（研究II）。此外，我们分析了阿孕瑞林（AP）、地塞米松（GC）或氯前列醇（CP）对牛胎盘小叶中HPGD表达的影响（研究III）。妊娠晚期（D272）和足月（NT）的组织用作对照。妊娠开始时和足月时，所有部位的HPGD含量最高。诱导分娩后，与足月时相比，阿孕瑞林和地塞米松处理后的HPGD含量较低，胎膜残留组和正常分娩胎膜组相似。胎盘小叶主要在胎儿部分染色；胎盘间信号见于子宫内膜腺上皮和腔上皮。结果表明，HPGD可能在妊娠建立和终止过程中起作用。
