Lee In Hong, Kim Hyun Soo, Seo Sang Heui
Laboratory of Influenza Research, College of Veterinary Medicine, Chungnam National University, 99 Dae-Hak Ro, Yuseong Gu, Daejeon, 305-764, Republic of Korea.
Institute of Influenza Virus, College of Veterinary Medicine, Chungnam National University, Daejeon, 305-764, Republic of Korea.
Arch Virol. 2017 Apr;162(4):1067-1071. doi: 10.1007/s00705-016-3216-x. Epub 2017 Jan 2.
Mast cells reside in many tissues, including the lungs, and might play a role in enhancing influenza virus infections in animals. In this study, we cultured porcine mast cells from porcine bone marrow cells with IL-3 and stem cell factor to study the infectivity and activation of the 2009 pandemic H1N1 influenza virus of swine origin. Porcine mast cells were infected with H1N1 influenza virus, without the subsequent production of infectious viruses but were activated, as indicated by the release of histamines. Inflammatory cytokine- and chemokine-encoding genes, including IL-1α, IL-6, CXCL9, CXCL10, and CXCL11, were upregulated in the infected porcine mast cells. Our results suggest that mast cells could be involved in enhancing influenza-virus-mediated disease in infected animals.
肥大细胞存在于包括肺在内的许多组织中，可能在增强动物流感病毒感染方面发挥作用。在本研究中，我们用白细胞介素-3和干细胞因子从猪骨髓细胞中培养猪肥大细胞，以研究源自猪的2009年大流行H1N1流感病毒的感染性和激活情况。猪肥大细胞感染了H1N1流感病毒，随后未产生传染性病毒，但如组胺释放所示，细胞被激活。在感染的猪肥大细胞中，包括白细胞介素-1α、白细胞介素-6、CXC趋化因子配体9、CXC趋化因子配体10和CXC趋化因子配体11在内的炎症细胞因子和趋化因子编码基因上调。我们的结果表明，肥大细胞可能参与增强受感染动物中流感病毒介导的疾病。
