Imai H, Tamai G
Faculty of Pharmaceutical Sciences, Fukuyama University, Japan.
Biomed Chromatogr. 1989 Sep;3(5):192-5. doi: 10.1002/bmc.1130030503.
An HPLC method with direct sample injections and column switching was investigated for the analysis of drugs in tissue homogenates. The appropriate precolumn packings and analytical column packings were surveyed in order to obtain quantitative recovery. The use of a large bore end-fitting filter for the precolumn avoided the interference due to minute tissue particles. A minicolumn was used to trap the analyte or clean up the sample. The method was applicable to hydrophilic as well as hydrophobic drugs in liver, kidney or heart tissues, and has been extensively used in the determination of drugs in centrifugal cell fractions such as the nucleus, mitochondria and microsome.
研究了一种采用直接进样和柱切换的高效液相色谱法分析组织匀浆中的药物。为了获得定量回收率，对合适的预柱填料和分析柱填料进行了考察。预柱使用大口径末端过滤装置可避免微小组织颗粒的干扰。采用微型柱捕集分析物或净化样品。该方法适用于肝脏、肾脏或心脏组织中亲水性和疏水性药物的分析，已广泛应用于细胞核、线粒体和微粒体等离心细胞组分中药物的测定。
