使用电泳免疫测定法监测激素分泌。

Using Electrophoretic Immunoassay to Monitor Hormone Secretion.

作者信息

Lu Shusheng, Kennedy Robert T

机构信息

Department of Chemistry, University of Michigan, 930 N. University Avenue, Ann Arbor, MI, 48109, USA.

Department of Pharmacology, University of Michigan, 1150 W. Medical Center Drive, Ann Arbor, MI, 48109, USA.

出版信息

Methods Mol Biol. 2017;1547:57-67. doi: 10.1007/978-1-4939-6734-6_5.

DOI:10.1007/978-1-4939-6734-6_5

PMID:28044287

Abstract

It has been demonstrated that microfluidic systems allow integration of sampling, reagent mixing, and rapid electrophoretic analysis. They have also proven useful for culturing cells wherein control over the environment allows novel and automated experiments. Here, we describe a microchip-based electrophoresis assay that allows cell culture and hormone monitoring. An online gradient generator can control cell culture condition precisely. This system has been applied for Pancreas islets' glucose sensitivity studies.

摘要

已经证明，微流控系统允许集成采样、试剂混合和快速电泳分析。它们也已被证明可用于细胞培养，其中对环境的控制允许进行新颖的自动化实验。在这里，我们描述了一种基于微芯片的电泳分析方法，该方法允许细胞培养和激素监测。在线梯度发生器可以精确控制细胞培养条件。该系统已应用于胰岛的葡萄糖敏感性研究。