Cognart Hamizah A, Chang Chia-Pin
Institut Curie, PSL Research University, CNRS, UMR 168, 26 rue d'Ulm, 75005, Paris, France.
Sorbonne Universités, UPMC Univ Paris 06, CNRS, UMR 168, Paris, France.
Methods Mol Biol. 2017;1547:167-174. doi: 10.1007/978-1-4939-6734-6_13.
The enumeration and analysis of circulating tumor cells (CTCs) is an increasing interest for monitoring disease progression or response to treatment, specifically as a companion diagnostic for new anticancer drugs, and for research into the mechanisms of disease progression and metastases. Ideally, CTCs would be enriched from very small samples, with minimal handling, high recovery, and no requirement for the expression of specific surface markers. Here, we describe negative enrichment as the preferred approach for cancer cell isolation using a microfluidic platform.
循环肿瘤细胞（CTC）的计数与分析对于监测疾病进展或治疗反应愈发重要，特别是作为新型抗癌药物的伴随诊断方法，以及用于研究疾病进展和转移的机制。理想情况下，CTC应能从非常小的样本中富集，操作最少，回收率高，且无需特定表面标志物的表达。在此，我们描述了使用微流控平台进行癌细胞分离时，阴性富集作为首选方法。
