De Angelis F, Ceccarelli S, Viscomi G C, Pinori M, Verdini A S
Dipartimento di Chimica, Università La Sapienza, Roma, Italy.
Biomed Environ Mass Spectrom. 1989 Oct;18(10):867-71. doi: 10.1002/bms.1200181005.
Fast atom bombardment (FAB) mass spectrometry has been successfully applied to the analysis of partially modified retro-inverso peptide isomers. The spectra are characterized by abundant protonated molecular ions and also by sequence ions due to fragmentation of the inverted bonds. Unambiguous information, as to the nature and the position in the backbone of the amino acids involved in the partial modification of the structure, are given by using a combination of FAB mass spectrometry and partial, selective acid hydrolysis, without separation of the resulting peptide mixtures.
快原子轰击（FAB）质谱法已成功应用于部分修饰的逆序肽异构体的分析。该光谱的特征在于有大量的质子化分子离子，以及由于反向键断裂而产生的序列离子。通过结合使用FAB质谱法和部分选择性酸水解，无需分离所得的肽混合物，就能给出有关参与结构部分修饰的氨基酸的性质及其在主链中位置的明确信息。
