Closset J, Dombrowicz D, Vandenbroeck M, Scippo M L, Igout A, Gothot A, Sente B, Hennen G
Bull Mem Acad R Med Belg. 1989;144(1-2):188-96; discussion 196-7.
We have studied the specific effects of highly purified pituitary hormones on testicular cell multiplication and differentiation in the immature hypophysectomized rat. LH exhibits multiplicative effects on Leydig cells and, curiously, regressive effects on germ cells. Seminiferous tubules are the main site of action of FSH which induces direct differentiation of Sertoli cells and paracrine effects on germ cells resulting in their multiplication to the pachytene stage (in the absence of androgen). Through interstitium-directed paracrine effects, FSH promotes the differentiation of Leydig cells. Prolactin and lactogenic growth hormones are capable of exerting both differentiative and multiplicative effects on Leydig cells. The purity of the hormone preparations permit to define their specific and direct effects and those mediated by paracrine or autocrine factors synthetized locally under their control. Among these potential factors, we have demonstrated the pituitary-dependent expression of IGF-I, c-myc, c-fos proteins. The purity of the preparations used in this work permits to distinguish clearly the specific and direct effect of each pituitary hormone from those mediated by paracrine or autocrine factors.
我们研究了高纯度垂体激素对未成熟去垂体大鼠睾丸细胞增殖和分化的特定影响。促黄体生成素（LH）对睾丸间质细胞有增殖作用，奇怪的是，对生殖细胞有退化作用。曲细精管是促卵泡生成素（FSH）的主要作用部位，FSH可诱导支持细胞直接分化，并对生殖细胞产生旁分泌作用，使其增殖至粗线期（在无雄激素的情况下）。通过间质定向旁分泌作用，FSH促进睾丸间质细胞的分化。催乳素和生乳生长激素对睾丸间质细胞具有分化和增殖作用。激素制剂的纯度能够确定其特定的直接作用以及由其控制下局部合成的旁分泌或自分泌因子介导的作用。在这些潜在因子中，我们已经证实了胰岛素样生长因子-I（IGF-I）、原癌基因c-myc、原癌基因c-fos蛋白的垂体依赖性表达。本研究中所用制剂的纯度能够清楚地区分每种垂体激素的特定直接作用与由旁分泌或自分泌因子介导的作用。
