Lilge Inga, Jiang Siyu, Schönherr Holger
Physical Chemistry I, Department of Chemistry and Biology and Research Center of Micro and Nanochemistry and Engineering (Cμ), University of Siegen, Adolf-Reichwein-Str. 2, 57076, Siegen, Germany.
Macromol Biosci. 2017 May;17(5). doi: 10.1002/mabi.201600451. Epub 2017 Jan 3.
The fabrication of 3D cell microenvironments exploiting versatile, long-term stable passivating poly(acryl amide) brushes in a microwell format and the study of the behavior of fibroblast and pancreatic tumor cells in wells of systematically varied shape and size is reported. The microwells, which are obtained by combining micromolding in capillaries with microcontact printing of initiator monolayers for subsequent surface-initiated polymerization of acrylamide and controlled functionalization with fibronectin (FN), expose cell adhesive areas inside the wells and protein and cell resistant brushes on the topside plateaus. NIH 3T3 fibroblast and pancreatic tumor (Patu 8988T) cells adhere and remain viable in the FN coated microwells for more than 1 week. Compared to 2D patterns, both cell lines are observed to attach to the bottom as well as the sidewalls of the microwells. The cytoskeleton alignment is found to be less pronounced compared to 2D patterned substrates, independent of microwell size and geometry.
报道了利用多功能、长期稳定的钝化聚（丙烯酰胺）刷以微孔形式构建3D细胞微环境，以及研究成纤维细胞和胰腺肿瘤细胞在形状和大小系统变化的微孔中的行为。这些微孔是通过将毛细管微成型与引发剂单层的微接触印刷相结合而获得的，用于随后丙烯酰胺的表面引发聚合以及用纤连蛋白（FN）进行可控功能化，微孔内部暴露细胞粘附区域，顶部平台上有蛋白质和细胞抗性刷。NIH 3T3成纤维细胞和胰腺肿瘤（Patu 8988T）细胞在FN包被的微孔中粘附并存活超过1周。与2D模式相比，观察到两种细胞系都附着在微孔的底部以及侧壁上。与2D图案化底物相比，细胞骨架排列不那么明显，与微孔大小和几何形状无关。
