适用于自由活动大鼠的定量2-[14C]脱氧葡萄糖法。

Adaptation of the quantitative 2-[14C]deoxyglucose method for use in freely moving rats.

作者信息

Crane A M, Porrino L J

机构信息

Laboratory of Cerebral Metabolism, National Institute of Mental Health, Bethesda, MD 20892.

出版信息

Brain Res. 1989 Oct 9;499(1):87-92. doi: 10.1016/0006-8993(89)91137-2.

DOI:10.1016/0006-8993(89)91137-2

PMID:2804673

Abstract

A procedure for venous and arterial catheterization is described which allows the quantitative 2-[14C]deoxyglucose method to be applied to freely moving animals for behavioral and pharmacological studies. The catheterization method is rapid, minimally invasive, and requires no complicated equipment. Physiological conditions and rates of cerebral glucose utilization in freely moving rats and in restrained rats have been compared. The results demonstrate that local cerebral glucose utilization can readily be measured in freely moving animals engaged in behavioral experiments.

摘要

本文描述了一种静脉和动脉插管程序，该程序可将定量2-[¹⁴C]脱氧葡萄糖法应用于自由活动的动物，以进行行为学和药理学研究。插管方法快速、微创，且无需复杂设备。已比较了自由活动大鼠和束缚大鼠的生理状况及脑葡萄糖利用速率。结果表明，在进行行为实验的自由活动动物中，可以很容易地测量局部脑葡萄糖利用情况。