Ichimiya Y, Emson P C, Mountjoy C Q, Lawson D E, Iizuka R
MRC Group, AFRC Institute of Animal Physiology and Genetics Research, Babraham, Cambridge, U.K.
Brain Res. 1989 Oct 16;499(2):402-6. doi: 10.1016/0006-8993(89)90793-2.
An antibody to the calcium binding protein, calbindin D28K (CaBP), was used to study the number and size of CaBP-immunoreactive neurones in the nucleus basalis of Meynert (nbM) of postmortem human brains from neurologically normal controls and from patients with neuropathologically diagnosed Alzheimer-type dementia (ATD). In controls, almost all the large neurones and their processes in the nbM were CaBP immunoreactive. Compared to neurologically normal controls the number of CaBP-immunoreactive neurones in the nbM in patients dying with ATD was significantly reduced and there was a clear loss of the majority of CaBP immunoreactive neurones. The few remaining nbM CaBP immunoreactive neurones in the ATD cases were smaller than those in the neurologically normal controls. Double-staining experiments revealed that many of the nbM CaBP-immunoreactive neurones contained choline acetyltransferase immunoreactivity, so that CaBP is an alternative marker for the nbM cholinergic neurones in the human fore-brain. These findings suggest that a disturbance in calcium homeostasis may be a possible factor contributing to the loss of these cholinergic/CaBP-containing neurones.
利用一种针对钙结合蛋白钙结合蛋白D28K（CaBP）的抗体，研究了来自神经功能正常对照组和经神经病理学诊断为阿尔茨海默型痴呆（ATD）患者的死后人类大脑Meynert基底核（nbM）中CaBP免疫反应性神经元的数量和大小。在对照组中，nbM中几乎所有的大神经元及其突起都具有CaBP免疫反应性。与神经功能正常的对照组相比，死于ATD的患者nbM中CaBP免疫反应性神经元的数量显著减少，并且大多数CaBP免疫反应性神经元明显丧失。ATD病例中剩余的少数nbM CaBP免疫反应性神经元比神经功能正常的对照组中的神经元小。双重染色实验表明，许多nbM CaBP免疫反应性神经元含有胆碱乙酰转移酶免疫反应性，因此CaBP是人类前脑nbM胆碱能神经元的一种替代标记物。这些发现表明，钙稳态紊乱可能是导致这些含胆碱能/CaBP神经元丧失的一个可能因素。
