Bunting T A, Scott B S
Department of Physiology, John Hopkins School of Medicine, Baltimore, MD.
Brain Res. 1989 Oct 30;501(1):105-15. doi: 10.1016/0006-8993(89)91032-9.
The effect of age of donor mouse and acute ethanol exposure on the electrical membrane properties (EMP) of 825 freshly dissociated dorsal root ganglion (DRG) neurons was investigated. Both age and ethanol exerted a number of significant linear and independent effects. Age effects included a 52% increase in action potential overshoot, a 50% increase in a measure of afterhyperpolarization duration, a 31% increase in action potential duration due to decreased rate of repolarization and depolarization, and decreased electrical excitability. Also, specific membrane resistance increased and specific membrane capacitance decreased while the membrane time constant was not significantly affected by age. These EMP alterations with age for freshly dissociated DRG neurons are consistent with those reported previously for cultured DRG neurons and hence further support the hypothesis that the EMP of neurons in situ change significantly with age. Acute ethanol exposure caused relatively few EMP alterations including decreased electrical excitability, specific membrane capacitance, time constant, and overshoot; specific membrane resistance was increased. These changes are similar to those seen previously and are consistent with a membrane expansion effect of ethanol.
研究了供体小鼠年龄和急性乙醇暴露对825个新鲜解离的背根神经节（DRG）神经元电膜特性（EMP）的影响。年龄和乙醇都产生了许多显著的线性且独立的效应。年龄效应包括动作电位超射增加52%，后超极化持续时间的测量值增加50%，由于复极化和去极化速率降低导致动作电位持续时间增加31%，以及电兴奋性降低。此外，比膜电阻增加，比膜电容减小，而膜时间常数不受年龄的显著影响。新鲜解离的DRG神经元随年龄出现的这些EMP改变与先前报道的培养DRG神经元的改变一致，因此进一步支持了原位神经元的EMP随年龄显著变化的假说。急性乙醇暴露引起的EMP改变相对较少，包括电兴奋性降低、比膜电容、时间常数和超射降低；比膜电阻增加。这些变化与先前观察到的相似，并且与乙醇的膜扩张效应一致。
