Murthy S M, Goldschmidt R A, Rao L N, Ammirati M, Buchmann T, Scanlon E F
Department of Surgery, Evanston Hospital, Illinois 60201.
Cancer. 1989 Nov 15;64(10):2035-44. doi: 10.1002/1097-0142(19891115)64:10<2035::aid-cncr2820641012>3.0.co;2-l.
Influence of surgical trauma on experimental metastasis in healing wounds is investigated using a transplantable murine mammary carcinoma cell line, TA3Ha. Intravenous injection of 10(5), 10(6), and 2 x 10(6) TA3Ha cells into syngeneic Strain A mice led to liver or kidney tumor development in none of the 96, ten, and ten mice tested, respectively. In contrast, injection of 10(5) cells into mice immediately after hepatic wedge resection performed using milliwatt carbon dioxide laser and electrocautery resulted in tumor formation at the site of trauma in 21/37 (57%) and 25/52 (48%) mice, (P less than 0.001) respectively. Similar results were obtained in mice subjected to partial nephrectomy using the laser (nine of 18) and electrocautery (eight of 13). These results clearly demonstrate that surgical trauma renders a nonprivileged organ susceptible to experimental metastasis formation, and that at least in this model both laser and electrocautery have similar effects. Tumor cell injection 1, 7, and 10 days posthepatic surgery resulted in 36%, 20%, and 0% tumor formation, respectively, indicating that the earlier events in wound healing support tumor implantation and/or growth better than those later on. Frequency of tumor formation at sites of trauma in the peritoneum induced by scalpel blade, laser, and electrocautery were 28%, 50% and 82%, respectively. Peritoneal tumors were seen in 33% of the nonsurgical mice. Skin incisions induced with the three above probes had little influence on experimental metastasis formation. Thus the influence of trauma on tumor formation is not uniform in every organ.
使用可移植的小鼠乳腺癌细胞系TA3Ha，研究手术创伤对愈合伤口实验性转移的影响。向同基因A系小鼠静脉注射10⁵、10⁶和2×10⁶个TA3Ha细胞，在分别测试的96只、10只和10只小鼠中，均未导致肝脏或肾脏肿瘤形成。相比之下，在使用毫瓦二氧化碳激光和电灼进行肝楔形切除术后立即向小鼠注射10⁵个细胞，分别在21/37（57%）和25/52（48%）的小鼠创伤部位导致肿瘤形成（P<0.001）。在使用激光（18只中的9只）和电灼（13只中的8只）进行部分肾切除的小鼠中也获得了类似结果。这些结果清楚地表明，手术创伤使一个非特权器官易于形成实验性转移，并且至少在这个模型中，激光和电灼具有相似的效果。肝手术后1天、7天和10天注射肿瘤细胞，肿瘤形成率分别为36%、20%和0%，表明伤口愈合早期事件比后期事件更有利于肿瘤植入和/或生长。手术刀刀片、激光和电灼诱导的腹膜创伤部位肿瘤形成频率分别为28%、50%和82%。33%的非手术小鼠出现腹膜肿瘤。用上述三种探针进行的皮肤切口对实验性转移形成影响很小。因此，创伤对肿瘤形成的影响在每个器官中并不一致。
