利用基于单细胞的基因表达分析来理解发育和干细胞。
Understanding development and stem cells using single cell-based analyses of gene expression.
作者信息
Kumar Pavithra, Tan Yuqi, Cahan Patrick
机构信息
Department of Biomedical Engineering, Institute for Cell Engineering, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA.
Department of Biomedical Engineering, Institute for Cell Engineering, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA
出版信息
Development. 2017 Jan 1;144(1):17-32. doi: 10.1242/dev.133058.
Abstract
In recent years, genome-wide profiling approaches have begun to uncover the molecular programs that drive developmental processes. In particular, technical advances that enable genome-wide profiling of thousands of individual cells have provided the tantalizing prospect of cataloging cell type diversity and developmental dynamics in a quantitative and comprehensive manner. Here, we review how single-cell RNA sequencing has provided key insights into mammalian developmental and stem cell biology, emphasizing the analytical approaches that are specific to studying gene expression in single cells.
摘要
近年来，全基因组分析方法已开始揭示驱动发育过程的分子程序。特别是，能够对数千个单个细胞进行全基因组分析的技术进步，为以定量和全面的方式编目细胞类型多样性和发育动态提供了诱人的前景。在这里，我们回顾单细胞RNA测序如何为哺乳动物发育和干细胞生物学提供了关键见解，重点介绍了专门用于研究单细胞基因表达的分析方法。
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