Alawad Abdullah O, Alharbi Sultan N, Alhazzaa Othman A, Alagrafi Faisal S, Alkhrayef Mohammad N, Alhamdan Ziyad A, Alenazi Abdullah D, Hammad Mohamed, Alyahya Sami A, AlJohi Hasan A, Alanazi Ibrahim O
National Center for Stem Cell Technology, King Abdulaziz City for Science and Technology (KACST), Riyadh, Kingdom of Saudi Arabia.
National Center for Stem Cell Technology, King Abdulaziz City for Science and Technology (KACST), Riyadh, Kingdom of Saudi Arabia.; SAAD Research and Development Center, Clinical Research Laboratory and Radiation Oncology, SAAD Specialist Hospital, Al Khobar, Kingdom of Saudi Arabia.
Bioinform Biol Insights. 2016 Dec 21;10:291-300. doi: 10.4137/BBI.S40782. eCollection 2016.
Krüppel-like factor 4 (KLF4) is a pluripotency transcription factor that helps in generating induced pluripotent stem cells (iPSCs). We sequenced for the first time the full coding sequence of KLF4 (cKLF4), which is also known as the Arabian camel. Bioinformatics analysis revealed the molecular weight and the isoelectric point of cKLF4 protein to be 53.043 kDa and 8.74, respectively. The predicted cKLF4 protein sequence shows high identity with some other species as follows: 98% with Bactrian camel and 89% with alpaca KLF4 proteins. A three-dimensional (3D) structure was built based on the available crystal structure of the KLF4 (mKLF4) of 82 residues (PDB: 2 WBS) and by predicting 400 residues using bioinformatics software. The comparison confirms the presence of the zinc finger domains in cKLF4 protein. Phylogenetic analysis showed that KLF4 from the Arabian camel is grouped with the Bactrian camel, alpaca, cattle, and pig. This study will help in the annotation of KLF4 protein and in generating camel-induced pluripotent stem cells (CiPSCs).
Krüppel样因子4（KLF4）是一种多能性转录因子，有助于诱导多能干细胞（iPSC）的生成。我们首次对KLF4（cKLF4）的完整编码序列进行了测序，cKLF4也被称为阿拉伯骆驼。生物信息学分析显示，cKLF4蛋白的分子量和等电点分别为53.043 kDa和8.74。预测的cKLF4蛋白序列与其他一些物种具有高度同源性，具体如下：与双峰驼的同源性为98%，与羊驼KLF4蛋白的同源性为89%。基于82个残基的KLF4（mKLF4）的现有晶体结构（PDB：2WBS）并使用生物信息学软件预测400个残基，构建了三维（3D）结构。比较结果证实了cKLF4蛋白中存在锌指结构域。系统发育分析表明，阿拉伯骆驼的KLF4与双峰驼、羊驼、牛和猪的KLF4归为一类。这项研究将有助于KLF4蛋白的注释以及骆驼诱导多能干细胞（CiPSC）的生成。
