Nagao Y, Tamatani R, Kawarai Y, Nishikawa K
Department of Biochemistry, Kanazawa Medical University, Ishikawa, Japan.
Cell Biol Int Rep. 1989 Oct;13(10):873-9. doi: 10.1016/0309-1651(89)90129-x.
Our recent studies indicated that Rhodamine fibrosarcoma (RdF) tissue of rats accumulated large amounts of albumin and transferrin and that these proteins were essential for growth of RdF cells in primary cultures in serum-free medium. Therefore, the localizations of albumin and transferrin accumulated in RdF tissue were examined by immunohistochemical stainings. Both anti-rat albumin IgG and anti-rat transferrin IgG stained the cell surface of the tumor cells strongly, but the intracellular area only weakly.
我们最近的研究表明，大鼠的若丹明纤维肉瘤（RdF）组织积累了大量白蛋白和转铁蛋白，并且这些蛋白质对于无血清培养基中原代培养的RdF细胞生长至关重要。因此，通过免疫组织化学染色检查了RdF组织中积累的白蛋白和转铁蛋白的定位。抗大鼠白蛋白IgG和抗大鼠转铁蛋白IgG均强烈染色肿瘤细胞的细胞表面，但细胞内区域染色较弱。
