从兔肾中分离近端肾小管细胞纯悬液：形态学、生物化学及代谢评估

Isolation of a pure suspension of proximal tubular cells from the rabbit kidney: morphological, biochemical and metabolic assessment.

作者信息

Toutain H, Vauclin-Jacques N, Fillastre J P, Morin J P

机构信息

Institut National de la Santé et de la Recherche Médicale U.295, U.E.R de médecine de Rouen, Saint-Etienne du Rouvray, France.

出版信息

Cell Biol Int Rep. 1989 Aug;13(8):701-10. doi: 10.1016/0309-1651(89)90046-5.

DOI:10.1016/0309-1651(89)90046-5

PMID:2805082

Abstract

A pure suspension of proximal tubule cells from the rabbit kidney was isolated by a new procedure, without proteolytic enzyme treatment. Electron microscopic examination revealed that these intact cells had long microvilli. All the marker enzymes located in the proximal tubule were higher in the isolated cells compared with renal cortex. Adenylate cyclase activity of the isolated cells was increased by PTH and NaF stimulations, while other hormones had minor effects. This isolated cell suspension showed a high respiration rate, a linear glucose production and a normal cell ATP level. All these results confirmed the isolation of viable proximal tubular cells with high metabolic capacities from the rabbit kidney.

摘要

采用一种新方法，在不进行蛋白水解酶处理的情况下，分离出了兔肾近端小管细胞的纯悬浮液。电子显微镜检查显示，这些完整细胞具有长微绒毛。与肾皮质相比，分离细胞中所有位于近端小管的标记酶含量更高。甲状旁腺激素（PTH）和氟化钠（NaF）刺激可提高分离细胞的腺苷酸环化酶活性，而其他激素的影响较小。这种分离的细胞悬浮液显示出高呼吸速率、线性葡萄糖生成和正常的细胞三磷酸腺苷（ATP）水平。所有这些结果证实，已从兔肾中分离出具有高代谢能力的活近端小管细胞。