Fàbregas Norma, Formosa-Jordan Pau, Ibañes Marta, Caño-Delgado Ana I
Department of Molecular Genetics, Centre for Research in Agricultural Genomics (CRAG) CSIC-IRTA-UAB-UB, Barcelona, Spain.
The Sainsbury Laboratory, University of Cambridge, Bateman Street, Cambridge, CB2 1LR, UK.
Methods Mol Biol. 2017;1544:3-19. doi: 10.1007/978-1-4939-6722-3_1.
The plant vascular system provides transport and mechanical support functions that are essential for suitable plant growth and development. In Arabidopsis thaliana (Arabidopsis), the vascular tissues at the shoot inflorescence stems are disposed in organized vascular bundles. The vascular patterning emergence and development within the shoot inflorescence stems is under the control of plant growth regulators (De Rybel et al., Nat Rev Mol Cell Biol 17:30-40, 2016; Caño-Delgado et al., Annu Rev Cell Dev Biol 26:605-637, 2010). By using a combined approach of experimental methods for vascular tissues visualization and quantification together with theoretical methods through mathematical and computational modeling, we have reported that auxin transport and brassinosteroid signaling play complementary roles in the formation of the periodic vascular patterning in the shoot (Ibañes et al., Proc Natl Acad Sci U S A 106:13630-13635, 2009; Fàbregas et al., Plant Signal Behav 5:903-906, 2010; Fàbregas et al., PLoS Genet 11:e1005183, 2015). Here, we report the methodology for the interdisciplinary analysis of the shoot vascular patterning in the plant model Arabidopsis into a handle procedure for visualization, quantification, data analysis, and modeling implementation.
植物维管系统提供运输和机械支撑功能，这些功能对于植物的正常生长和发育至关重要。在拟南芥中，地上部花序茎中的维管组织排列成有组织的维管束。地上部花序茎内维管模式的出现和发育受植物生长调节剂的控制（德里贝尔等人，《自然综述：分子细胞生物学》17:30 - 40，2016；卡尼奥 - 德尔加多等人，《细胞与发育生物学年度评论》26:605 - 637，2010）。通过将用于维管组织可视化和定量的实验方法与通过数学和计算建模的理论方法相结合，我们已经报道生长素运输和油菜素类固醇信号传导在地上部周期性维管模式的形成中发挥互补作用（伊瓦涅斯等人，《美国国家科学院院刊》106:13630 - 13635，2009；法夫雷加斯等人，《植物信号与行为》5:903 - 906，2010；法夫雷加斯等人，《公共科学图书馆·遗传学》11:e1005183，2015）。在这里，我们报告了将植物模型拟南芥中地上部维管模式的跨学科分析转化为可视化、定量、数据分析和建模实施的操作程序的方法。
