Saito Masato, Nurani Alif Meem, Kondo Yuki, Fukuda Hiroo
Department of Biological Sciences, Graduate School of Science, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo, 113-0033, Japan.
Methods Mol Biol. 2017;1544:59-65. doi: 10.1007/978-1-4939-6722-3_5.
Culture systems combined with molecular biological approaches have identified various key factors regulating vascular differentiation, which makes a great contribution to development of vascular biology. Recently, we established a novel culture system for xylem cell differentiation in the model plant Arabidopsis thaliana (Arabidopsis), in which ectopic xylem cells can be induced throughout leaf disks within 3-4 days. Here we describe detailed procedures of the culture system from sample preparation to vascular cell induction culture.
结合分子生物学方法的培养系统已经鉴定出多种调节血管分化的关键因素，这对血管生物学的发展做出了巨大贡献。最近，我们在模式植物拟南芥中建立了一种用于木质部细胞分化的新型培养系统，在该系统中，异位木质部细胞可在3-4天内在整个叶盘中被诱导形成。在这里，我们描述了从样品制备到血管细胞诱导培养的培养系统的详细步骤。
