Melnyk Charles W
The Sainsbury Laboratory, University of Cambridge, Bateman Street, Cambridge, CB2 1LR, UK.
Methods Mol Biol. 2017;1544:91-102. doi: 10.1007/978-1-4939-6722-3_9.
Plants have a remarkable ability to regenerate vascular tissue after damage or wounding. A striking example of this phenomenon is the cutting and rejoining of plants during the process of grafting, which humans have used for millennia. Here, I describe how to graft Arabidopsis seedlings and how to monitor the vascular reconnection process during wound healing using fluorescent dyes and fluorescent proteins. Furthermore, I describe how to visualize xylem formation during graft healing. These techniques are useful for studying how the vascular system regenerates and for better understanding the process of plant grafting.
植物在受损或受伤后具有显著的再生维管组织的能力。这种现象的一个显著例子是在嫁接过程中对植物进行切割和重新连接，人类已经使用了数千年。在这里，我描述了如何嫁接拟南芥幼苗，以及如何使用荧光染料和荧光蛋白监测伤口愈合过程中的维管重新连接过程。此外，我还描述了如何在嫁接愈合过程中观察木质部的形成。这些技术对于研究维管系统如何再生以及更好地理解植物嫁接过程很有用。
