使用CellProfiler分析和量化血管形态。

Using CellProfiler to Analyze and Quantify Vascular Morphology.

作者信息

Campbell Liam, Kumar Manoj, Turner Simon

机构信息

University of Manchester, Oxford Road, Manchester, UK.

出版信息

Methods Mol Biol. 2017;1544:179-189. doi: 10.1007/978-1-4939-6722-3_13.

DOI:10.1007/978-1-4939-6722-3_13

PMID:28050836

Abstract

Computational programs can be used in place of time-consuming, error-prone manual data collection. CellProfiler is a free, open source program that allows researchers to automate image analysis and collect large amounts of phenotypic data relatively easily. Here, we describe how to adapt CellProfiler to analyze cross sections of xylem tissue and use it to gather a variety of information on traits such as cell size, shape, and number. We provide step-by-step instructions to create a typical CellProfiler analysis pipeline, alongside explanations of important modules, options and parameters available to the user.

摘要

计算程序可用于取代耗时且容易出错的手动数据收集。CellProfiler是一个免费的开源程序，它使研究人员能够相对轻松地自动进行图像分析并收集大量表型数据。在这里，我们描述了如何调整CellProfiler以分析木质部组织的横截面，并使用它来收集有关细胞大小、形状和数量等特征的各种信息。我们提供了创建典型CellProfiler分析管道的分步说明，以及对用户可用的重要模块、选项和参数的解释。