Pediatrics Research Institute, Ministry of Education Key Laboratory of Child Development and Disorders, Children's Hospital of Chongqing Medical University, Chongqing 400014, China.
Department of Pediatrics, First Affiliated Hospital of China Medical University, Shenyang 110001, China.
Sci Rep. 2017 Jan 4;7:39889. doi: 10.1038/srep39889.
The incidence and mortality of acute lung injury (ALI)/acute respiratory distress syndrome (ARDS) are still very high, but stem cells show some promise for its treatment. Here we found that intratracheal administration of human umbilical cord-mesenchymal stem cells (UC-MSCs) significantly improved survival and attenuated the lung inflammation in lipopolysaccharide (LPS)-induced ALI mice. We also used the proteins-chip and bioinformatics to analyze interactions between UC-MSCs treatment and immune-response alternations of ALI mice. Then we demonstrated that UC-MSCs could inhibit the inflammatory response of mouse macrophage in ALI mice, as well as enhance its IL-10 expression. We provide data to support the concept that the therapeutic capacity of UC-MSCs for ALI was primarily through paracrine secretion, particularly of prostaglandin-E2 (PGE2). Furthermore, we showed that UC-MSCs might secrete a panel of factors including GM-CSF, IL-6 and IL-13 to ameliorate ALI. Our study suggested that UC-MSCs could protect LPS-induced ALI model by immune regulation and paracrine factors, indicating that UC-MSCs should be a promising strategy for ALI/ARDS.
急性肺损伤（ALI）/急性呼吸窘迫综合征（ARDS）的发病率和死亡率仍然很高，但干细胞在其治疗方面显示出了一些希望。在这里，我们发现气管内给予人脐带间充质干细胞（UC-MSCs）可显著提高存活率并减轻脂多糖（LPS）诱导的 ALI 小鼠的肺部炎症。我们还使用蛋白质芯片和生物信息学分析了 UC-MSCs 治疗与 ALI 小鼠免疫反应改变之间的相互作用。然后，我们证明 UC-MSCs 可以抑制 ALI 中小鼠巨噬细胞的炎症反应，并增强其 IL-10 表达。我们提供的数据支持这样一种概念，即 UC-MSCs 治疗 ALI 的能力主要是通过旁分泌，特别是前列腺素 E2（PGE2）。此外，我们表明 UC-MSCs 可能分泌一组因子，包括 GM-CSF、IL-6 和 IL-13，以改善 ALI。我们的研究表明，UC-MSCs 可以通过免疫调节和旁分泌因子来保护 LPS 诱导的 ALI 模型，这表明 UC-MSCs 应该是 ALI/ARDS 的一种有前途的策略。
