Kim Hong-Seok, Choi Dasom, Kang Il-Byeong, Kim Dong-Hyeon, Yim Jin-Hyeok, Kim Young-Ji, Chon Jung-Whan, Oh Deog-Hwan, Seo Kun-Ho
1 Center for One Health, College of Veterinary Medicine, Konkuk University , Seoul, South Korea .
2 Department of Food Science and Biotechnology, School of Bioconvergence Science and Technology, Kangwon National University , Chuncheon, Gangwon, South Korea .
Foodborne Pathog Dis. 2017 Feb;14(2):84-88. doi: 10.1089/fpd.2016.2198. Epub 2017 Jan 4.
Culture-based detection of nontyphoidal Salmonella spp. in foods requires at least four working days; therefore, new detection methods that shorten the test time are needed. In this study, we developed a novel single-step Salmonella enrichment broth, SSE-1, and compared its detection capability with that of commercial single-step ONE broth-Salmonella (OBS) medium and a conventional two-step enrichment method using buffered peptone water and Rappaport-Vassiliadis soy broth (BPW-RVS). Minimally processed lettuce samples were artificially inoculated with low levels of healthy and cold-injured Salmonella Enteritidis (10 or 10 colony-forming unit/25 g), incubated in OBS, BPW-RVS, and SSE-1 broths, and streaked on xylose lysine deoxycholate (XLD) agar. Salmonella recoverability was significantly higher in BPW-RVS (79.2%) and SSE-1 (83.3%) compared to OBS (39.3%) (p < 0.05). Our data suggest that the SSE-1 single-step enrichment broth could completely replace two-step enrichment with reduced enrichment time from 48 to 24 h, performing better than commercial single-step enrichment medium in the conventional nonchromogenic Salmonella detection, thus saving time, labor, and cost.
基于培养法检测食品中的非伤寒沙门氏菌属需要至少四个工作日；因此，需要新的缩短检测时间的方法。在本研究中，我们开发了一种新型的单步沙门氏菌增菌肉汤SSE-1，并将其检测能力与市售的单步ONE肉汤-沙门氏菌（OBS）培养基以及使用缓冲蛋白胨水和Rappaport-Vassiliadis大豆肉汤（BPW-RVS）的传统两步增菌法进行了比较。将轻度加工的生菜样品用低水平的健康和冷损伤肠炎沙门氏菌（10或10个菌落形成单位/25克）人工接种，在OBS、BPW-RVS和SSE-1肉汤中培养，然后划线接种在木糖赖氨酸脱氧胆酸盐（XLD）琼脂上。与OBS（39.3%）相比，BPW-RVS（79.2%）和SSE-1（83.3%）中的沙门氏菌回收率显著更高（p＜0.05）。我们的数据表明，SSE-1单步增菌肉汤可以完全替代两步增菌法，将增菌时间从48小时缩短至24小时，在传统的非显色沙门氏菌检测中比市售单步增菌培养基表现更好，从而节省时间、人力和成本。
