Mamiya M, Takahashi K, Eguchi S, Morisaki M
Chem Pharm Bull (Tokyo). 1989 Jul;37(7):1930-1. doi: 10.1248/cpb.37.1930.
In order to establish the functional importance of the 10-methyl group of cholesterol and the planarity of the steroid ring, silkworms (Bombyx mori) were reared on an artificial diet containing 19-norcholesterol (1), 14 alpha-methylcholesterol (3) or 19,19-difluorocholesterol (2). The former two sterols (1 and 3) only partially satisfied the silkworm sterol requirement; growth and development were seriously retarded. The fluorinated sterol (2) was much more deleterious and was totally inadequate in meeting the sterol requirement.
为了确定胆固醇10 - 甲基基团的功能重要性以及甾体环的平面性，将家蚕（Bombyx mori）饲养在含有19 - 去甲胆固醇（1）、14α - 甲基胆固醇（3）或19,19 - 二氟胆固醇（2）的人工饲料上。前两种甾醇（1和3）仅部分满足家蚕对甾醇的需求；生长和发育严重受阻。氟化甾醇（2）的危害更大，完全不足以满足甾醇需求。
