Morii Sotokichi
Department of Pathology, Kansai Medical University, Moriguchi, Osaka 570, Japan (OA 82-2 70S2).
J Histochem Cytochem. 1983 Jan;31(1A_suppl):150-153. doi: 10.1177/31.1A_SUPPL.6827071.
The penetration of Rbizopus arrhizus lipase into ethioine fatty livers was examined histochemically. A free-floating, 30 μm thick Vibratome section of the livers perfused with aldehydes was shown to be suitable for ultracytochemical analysis by the lipase digestive method. Accessibility of phospholipids in the outer membrane of erythrocytes to Naja naja phospholipase A was observed under a light microscope, and intact red cells were used for the ultracytochemical analysis by the phospholipase digestive method. Ultracytochemical pictures of cytoplasmic lipids in these materials subjected to the enzymatic digestive method are presented.
用组织化学方法检测了米根霉脂肪酶对乙硫氨酸诱导的脂肪肝的渗透情况。结果表明，用醛灌注的肝脏制成的30μm厚的振动切片机自由漂浮切片，适用于脂肪酶消化法的超微细胞化学分析。在光学显微镜下观察了眼镜蛇磷脂酶A对红细胞外膜中磷脂的可及性，并将完整的红细胞用于磷脂酶消化法的超微细胞化学分析。本文展示了采用酶消化法处理这些材料后，其细胞质脂质的超微细胞化学图片。
