Buszewski Bogusław, Railean-Plugaru Viorica, Pomastowski Pawel, Rafinska Katarzyna, Szultka-Mlynska Malgorzata, Kowalkowski Tomasz
Interdisciplinary Centre of Modern Technology, Nicolaus Copernicus University, Wileńska 4, 87-100 Torun, Poland.
Curr Pharm Biotechnol. 2017;18(2):168-176. doi: 10.2174/1389201018666170104112434.
Biologically synthetized silver nanoparticles are promising antimicrobial agent. Flow cytometry, well diffusion methods, colony-forming units (CFU) and spectroscopic approach are commonly used in antimicrobial study. The aim of this study was investigation of effectiveness of Bio- AgNPs synthesized by Actinomycetes HGG16n using fluorescence flow cytometry method as an alternative to the standard ones (well and disc diffusion method).
Flow cytometry technique was applied to monitor the antibacterial effect of biocolloidal silver and its combination with various commercial antibiotics against selected pathogens. The observed effectiveness was confirmed by fluorescence micrographs.
Silver nanoparticles synthesized by Actinomycetes HGG 16n strain were as effective antimicrobial agent as the tested commercial antibiotics. Escherichia coli, Klebsiella pneumoniae, Proteus mirabilis, Salmonella infantis and Bacillus subtilis strains are not able to create the resistant mechanisms under treatment of biocolloidal silver. Furthermore, the flow cytometry technique was more sensitive than disc and well diffusion and confirmed the effectiveness of BioAgNPs against all tested bacterial cells. Precipitation and limited diffusion of biocolloidal silver was observed by using well diffusion method.
Specificity and selection of antimicrobial approach are related with different nature of lowmolecular compounds (e.g. antibiotic) compared with biocolloids. An alternative method, flow cytometry was designed for antimicrobial study of biocolloidal silver nanoparticles and compared to the classical microbial techniques. Moreover, this work highlights the development of novel and inexpensive antimicrobial agent for most oral and skin infections caused by Gram (+) and Gram (-) bacteria.
生物合成的银纳米颗粒是很有前景的抗菌剂。流式细胞术、平板扩散法、菌落形成单位（CFU）和光谱法常用于抗菌研究。本研究的目的是使用荧光流式细胞术方法研究放线菌HGG16n合成的生物银纳米颗粒（Bio-AgNPs）的有效性，作为标准方法（平板和纸片扩散法）的替代方法。
应用流式细胞术技术监测生物胶体银及其与各种市售抗生素组合对选定病原体的抗菌效果。通过荧光显微照片证实观察到的有效性。
放线菌HGG 16n菌株合成的银纳米颗粒作为抗菌剂与测试的市售抗生素一样有效。大肠杆菌、肺炎克雷伯菌、奇异变形杆菌、婴儿沙门氏菌和枯草芽孢杆菌菌株在生物胶体银处理下无法产生耐药机制。此外，流式细胞术技术比纸片和平板扩散法更敏感，并证实了BioAgNPs对所有测试细菌细胞的有效性。使用平板扩散法观察到生物胶体银的沉淀和有限扩散。
与生物胶体相比，抗菌方法的特异性和选择与低分子化合物（如抗生素）的不同性质有关。设计了一种替代方法——流式细胞术，用于生物胶体银纳米颗粒的抗菌研究，并与经典微生物技术进行比较。此外，这项工作突出了开发新型且廉价的抗菌剂用于治疗由革兰氏阳性菌和革兰氏阴性菌引起的大多数口腔和皮肤感染。
