新型德氏固氮链霉菌产生的生物银纳米粒子的抗菌活性。
Antimicrobial activity of biosilver nanoparticles produced by a novel Streptacidiphilus durhamensis strain.
机构信息
Department of Environmental Chemistry and Bioanalytics, Faculty of Chemistry, Nicolaus Copernicus University, Torun, Poland; Interdisciplinary Centre of Modern Technology, Nicolaus Copernicus University, Torun, Poland.
Department of Environmental Chemistry and Bioanalytics, Faculty of Chemistry, Nicolaus Copernicus University, Torun, Poland; Interdisciplinary Centre of Modern Technology, Nicolaus Copernicus University, Torun, Poland.
出版信息
J Microbiol Immunol Infect. 2018 Feb;51(1):45-54. doi: 10.1016/j.jmii.2016.03.002. Epub 2016 Mar 31.
BACKGROUND/PURPOSE: In this study, an acidophilic actinobacteria strain was used as a novel reducing agent for a single-step synthesis of nanostructure silver particles. We used a Streptacidiphilus durhamensis HGG16n isolate for efficient synthesis of bioactive silver nanoparticles [bio(AgNPs)] in an inexpensive, eco-friendly, and nontoxic manner. The obtained bio(AgNPs) exhibited unique physicochemical and biochemical properties.
METHODS
Structural, morphological, and optical properties of the synthesized biocolloids were characterized by spectroscopy, dynamic light scattering, and electron microscopy approaches. The antimicrobial activity was evaluated using the well- and disc-diffusion methods.
RESULTS
The obtained crystalline structure and stable biosynthesized silver nanoparticles ranged in size from 8 nm to 48 nm and were mostly spherical in shape. Antimicrobial assays of the silver nanoparticles against pathogenic bacteria showed the highest antimicrobial activity against Pseudomonas aeruginosa, Staphylococcus aureus, and Proteus mirabilis, followed by Escherichia coli, Klebsiella pneumoniae, and Bacillus subtilis. Moreover, the synergistic effect of bio(AgNPs) with various commercially available antibiotics was also evaluated.
CONCLUSION
These results provide insight into the development of new antimicrobial agents along with synergistic enhancement of the antibacterial mechanism against clinical bacteria.
背景/目的：在这项研究中，一种嗜酸放线菌菌株被用作一种新的还原剂，用于一步法合成纳米结构银颗粒。我们使用一株嗜热链球菌 HGG16n 分离株，以一种廉价、环保和无毒的方式高效合成具有生物活性的银纳米粒子[生物（AgNPs）]。所获得的生物（AgNPs）表现出独特的物理化学和生化性质。
方法
通过光谱学、动态光散射和电子显微镜方法对合成的生物胶体的结构、形态和光学性质进行了表征。采用平板和圆盘扩散法评估了抗菌活性。
结果
所得的结晶结构和稳定的生物合成银纳米粒子的尺寸范围为 8nm 至 48nm，形状大多为球形。银纳米粒子对致病菌的抗菌试验表明，对铜绿假单胞菌、金黄色葡萄球菌和奇异变形杆菌的抗菌活性最高，其次是大肠杆菌、肺炎克雷伯菌和枯草芽孢杆菌。此外，还评估了生物（AgNPs）与各种市售抗生素的协同作用。
结论
这些结果为开发新的抗菌剂以及协同增强抗菌机制对抗临床细菌提供了深入了解。
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