Zhu Xinyu, Treu Laura, Kougias Panagiotis G, Campanaro Stefano, Angelidaki Irini
Department of Environmental Engineering, Technical University of Denmark, Kgs. Lyngby, DK-2800, Denmark.
Department of Environmental Engineering, Technical University of Denmark, Kgs. Lyngby, DK-2800, Denmark; Department of Agronomy, Food, Natural Resources, Animal and Environment (DAFNAE), University of Padova, Viale dell'Università, 16, 35020, Legnaro, Padova, Italy.
Anaerobe. 2017 Aug;46:69-77. doi: 10.1016/j.anaerobe.2016.12.015. Epub 2017 Jan 3.
Upflow anaerobic sludge blanket (UASB) technology refers to reactor technology where granules, i.e. self-immobilised microbial associations, are the biological catalysts involved in the anaerobic digestion process. During the start-up period, UASB reactors operate at relatively long HRT and therefore the liquid phase of the reactor becomes a favourable environment for microbial growth. The current study aimed to elucidate the dynamicity of the suspended microbial community in UASB reactors, during the transition from mesophilic to thermophilic conditions. High throughput 16S rRNA amplicon sequencing was used to characterize the taxonomic composition of the microbiome. The results showed that the microbial community was mainly composed by hydrolytic and fermentative bacteria. Results revealed relevant shifts in the microbial community composition, which is mainly determined by the operational conditions and the reactor performance. Finally, shared OTUs between the microbial consortia of the suspended and the granular sludge showed that planktonic microbiota is significantly influencing the granule microbial community composition.
上流式厌氧污泥床（UASB）技术是指一种反应器技术，其中颗粒，即自我固定化的微生物群落，是厌氧消化过程中涉及的生物催化剂。在启动阶段，UASB反应器在相对较长的水力停留时间（HRT）下运行，因此反应器的液相成为微生物生长的有利环境。当前的研究旨在阐明UASB反应器中悬浮微生物群落从嗜温条件转变为嗜热条件期间的动态变化。高通量16S rRNA扩增子测序用于表征微生物群落的分类组成。结果表明，微生物群落主要由水解和发酵细菌组成。结果显示微生物群落组成发生了相关变化，这主要由运行条件和反应器性能决定。最后，悬浮污泥和颗粒污泥的微生物群落之间共享的操作分类单元（OTU）表明，浮游微生物群对颗粒微生物群落组成有显著影响。
