Morais G B, Viana D A, Silva F M O, Xavier Júnior F A F, Farias K M, Pessoa C D'Ó, Silveira J A M, Alves A P N N, Mota M R L, Silva F D O, Sampaio C M S, Verdugo J M G, Evangelista J S A M
Faculdade de Veterinária, Universidade Estadual do Ceará, Ceará, Brazil.
Departamento de Fisiologia e Farmacologia, Universidade Federal do Ceará, Ceará, Brazil.
Microsc Res Tech. 2017 May;80(5):543-550. doi: 10.1002/jemt.22830. Epub 2017 Jan 6.
Chronic kidney disease (CKD) is a relevant disease in feline clinic. The tubulointerstitial damage, with collagen deposition and fibrosis, is an important result of this process. The aim of this study was to quantify and correlate the deposition of collagen and severity of interstitial fibrosis (IF) in the kidney from cats in different stages of CKD. Kidney fragments from 10 adult cats with CKD were analyzed and stained by Masson's trichrome (MT) and Picrosirius red (PSR) for circular polarized microscopy. Random quantitative analysis was performed on MT sections to classify the degree of IF, per field area, with and without circular polarization. Statistics correlations were performed by Spearman's (ρ; p < .05). There was a significant correlation of IF quantification with the area of interstitial collagen deposition by polarized PSR (PSRp) (r = .7939, p = .0098) and nonpolarized PSR (PSRn) (r = .7781, p = .0080). There was a positive correlation of serum creatinine (sCr) at different stages of CKD with PSRp (r = .7939, p = .0098), PSRn (r = .8667, p = .0027) and MT (r = .7818, p = .0117). Correlations between the percentage of quantified area was also positive from PSRp to PSRn (r = .9030, p = .0009) and PSRp to MT (r = .7939, p = .0098). The PSRN was also correlated with MT (r = .9273, p = .0001). The correlation with IF and sCr follows the disease evolution and the quantification of collagen by PSR is an excellent tool for analyzing the disease severity at different stages.
慢性肾脏病（CKD）是猫科动物临床中的一种相关疾病。伴有胶原蛋白沉积和纤维化的肾小管间质损伤是这一过程的重要结果。本研究的目的是量化并关联不同CKD阶段猫肾脏中胶原蛋白的沉积与间质纤维化（IF）的严重程度。对10只患有CKD的成年猫的肾脏组织切片进行分析，并用Masson三色染色法（MT）和苦味酸天狼星红染色法（PSR）进行染色，用于圆偏振显微镜观察。对MT切片进行随机定量分析，以根据视野面积对有无圆偏振的IF程度进行分类。采用Spearman秩相关分析（ρ；p <.05）。IF定量与偏振PSR（PSRp）（r =.7939，p =.0098）和非偏振PSR（PSRn）（r =.7781，p =.0080）的间质胶原沉积面积显著相关。CKD不同阶段的血清肌酐（sCr）与PSRp（r =.7939，p =.0098）、PSRn（r =.8667，p =.0027）和MT（r =.7818，p =.0117）呈正相关。定量面积百分比之间从PSRp到PSRn（r =.9030，p =.0009）和PSRp到MT（r =.7939，p =.0098）也呈正相关。PSRN与MT也相关（r =.9273，p =.0001）。IF与sCr的相关性遵循疾病进展，PSR对胶原蛋白的定量是分析不同阶段疾病严重程度的优秀工具。
