Key Laboratory of Chemistry of Northwestern Plant Resources, Chinese Academy of Sciences, Lanzhou, P.R. China.
Key Laboratory for Natural Medicine of Gansu Province, Lanzhou Institute of Chemical Physics, Chinese Academy of Sciences, Lanzhou, P.R. China.
J Sep Sci. 2017 Mar;40(6):1403-1409. doi: 10.1002/jssc.201601238. Epub 2017 Feb 22.
As a novel solid-phase extraction material, zinc sulfide nanosheets were prepared by a simple method and were used to extract flavonoids. We used scanning electron microscopy to show its nanosheet morphology and energy dispersive X-ray spectroscopy and powder X-ray diffraction to confirm its chemical and phase compositions. Coupled to a high-performance liquid chromatography, the zinc sulfide nanosheets were packed into a microcolumn and were used to extract four model flavonoids to examine their extraction ability. The parameters of sample loading and elution were investigated. Under optimized conditions, the analytical method for flavonoids was established. For the method, wide linearities from 1 to 250 μg/L and low limits of detection from 0.25 to 0.5 μg/L were obtained. The relative standard deviations for single column repeatability and column to column reproducibility were less than 7.7 and 10.4%, respectively. The established method was also used to analyze two real samples and the recoveries from 88.7 to 98.2% further proved the reliability of the method. Moreover, the zinc sulfide nanosheets have good stability and that in one column can be reused for more than 50 times. This work proves that the prepared zinc sulfide nanosheets are a good candidate as the flavonoids sorbent.
作为一种新型的固相萃取材料，通过简单的方法制备了硫化锌纳米片，并将其用于提取黄酮类化合物。我们使用扫描电子显微镜展示了其纳米片形貌，使用能量色散 X 射线光谱和粉末 X 射线衍射法确认了其化学和相组成。将硫化锌纳米片耦合到高效液相色谱中，将其填充到微柱中用于提取四种模型黄酮类化合物，以考察其提取能力。研究了样品加载和洗脱的参数。在优化条件下，建立了黄酮类化合物的分析方法。对于该方法，获得了从 1 到 250 μg/L 的宽线性范围和从 0.25 到 0.5 μg/L 的低检测限。单个柱重复性和柱间重现性的相对标准偏差分别小于 7.7%和 10.4%。该方法还用于分析两个实际样品，回收率在 88.7%至 98.2%之间，进一步证明了该方法的可靠性。此外，硫化锌纳米片具有良好的稳定性，一个柱中的纳米片可以重复使用 50 多次以上。这项工作证明，所制备的硫化锌纳米片是一种良好的黄酮类化合物吸附剂。
