Toombs Jamie, Foiani Martha S, Paterson Ross W, Heslegrave Amanda, Wray Selina, Schott Jonathan M, Fox Nick C, Lunn Michael P, Blennow Kaj, Zetterberg Henrik
Department of Molecular Neuroscience, Institute of Neurology, University College London, London, UK.
Department of Neurodegeneration, Dementia Research Centre, Institute of Neurology, London, UK.
J Alzheimers Dis. 2017;56(3):885-891. doi: 10.3233/JAD-161126.
The effect of spinal manometers on cerebrospinal fluid (CSF) amyloid-β (Aβ) concentration was investigated. Pooled human CSF samples were divided in two, one half passed through a manometer into a collection tube, the other transferred directly to a collection tube. CSF was analyzed for Aβ38/40/42 using an electrochemiluminescence immunoassay. Relative to control, use of a manometer decreased Aβ38/40/42 concentration by 5.6% (±1.5SE), 4.4% (±1.7SE), and 4.3% (±2.4SE), respectively. The ratios of Aβ42 :40, Aβ42 :38, and Aβ40 :38 were not affected by manometer treatment. Factors which artificially lower CSF Aβ concentrations are relevant to clinical diagnosis for AD and study design.
研究了脊髓压力计对脑脊液（CSF）淀粉样β蛋白（Aβ）浓度的影响。将合并的人类脑脊液样本分成两份，其中一半通过压力计流入收集管，另一半直接转移至收集管。采用电化学发光免疫分析法对脑脊液中的Aβ38/40/42进行分析。与对照组相比，使用压力计后Aβ38/40/42浓度分别降低了5.6%（±1.5标准误）、4.4%（±1.7标准误）和4.3%（±2.4标准误）。压力计处理对Aβ42:40、Aβ42:38和Aβ40:38的比例没有影响。人为降低脑脊液Aβ浓度的因素与阿尔茨海默病的临床诊断和研究设计相关。
