使用合成肽和抗合成肽抗体通过放射免疫测定法在溶解的人胎盘膜中证实两种不同的胰岛素结合成分。

Demonstration of two distinct insulin-binding components in solubilized human placental membranes by radioimmunoassay using synthetic peptide and anti-synthetic peptide antibody.

作者信息

Yanaihara N, Yanaihara C, Iguchi K, Inoue T, Toyoshige M, Mochizuki T, Hoshino M, Kurokawa N, Kaneko T

机构信息

University of Shizuoka School of Pharmaceutical Sciences, Japan.

出版信息

Diabetes Res Clin Pract. 1989;7 Suppl 1:S109-13. doi: 10.1016/0168-8227(89)90096-x.

DOI:10.1016/0168-8227(89)90096-x

PMID:2806053

Abstract

A peptide corresponding to the 957-980 sequence of human placental insulin receptor precursor (HIRP) was synthesized and antisera were produced against the synthetic peptide. Anti-synthetic HIRP(957-980) serum HIR-27 was proved to cross-react with HIRP-related proteins in solubilized human placental membranes. A radioimmunoassay developed with the antiserum and synthetic peptide HIRP(957-980) enabled us to separate, in combination with gel filtration, two insulin-binding components in solubilized human placental membranes which conceivably correspond to the alpha 2 beta 2 and alpha beta structures of the placental insulin receptor. The two components were shown to be distinct in insulin-binding behavior depending on conditions of pH and ionic strength in the binding assay.

摘要

合成了一段与人类胎盘胰岛素受体前体（HIRP）957 - 980序列相对应的肽，并针对该合成肽制备了抗血清。抗合成HIRP（957 - 980）血清HIR - 27被证明能与溶解的人胎盘膜中的HIRP相关蛋白发生交叉反应。用该抗血清和合成肽HIRP（957 - 980）开发的放射免疫测定法，使我们能够结合凝胶过滤，分离出溶解的人胎盘膜中的两种胰岛素结合成分，这两种成分可能分别对应胎盘胰岛素受体的α2β2和αβ结构。结果表明，在结合测定中，根据pH和离子强度条件，这两种成分在胰岛素结合行为上是不同的。