澳大利亚患者中肺炎链球菌血清群 35 变异株的发现。
Discovery of Streptococcus pneumoniae serogroup 35 variants in Australian patients.
机构信息
Public Health Microbiology, Forensic and Scientific Services, Queensland Department of Health, Coopers Plains, Queensland, Australia.
Public Health Microbiology, Forensic and Scientific Services, Queensland Department of Health, Coopers Plains, Queensland, Australia.
出版信息
Clin Microbiol Infect. 2017 Jul;23(7):476-479. doi: 10.1016/j.cmi.2016.12.029. Epub 2017 Jan 3.
OBJECTIVES
Streptococcus pneumoniae isolates from Australian invasive pneumococcal disease cases displaying an atypical 35B phenotype. Whole genome sequencing was used to analyse these strains and identify changes to the capsule gene regions.
METHODS
Four atypical serogroup 35 isolates from Australian reference laboratories were unable to be assigned to one of the four known group 35 serotypes by the Quellung serotyping method. Genetic characterization of the capsule locus was performed by bioinformatic analysis of whole genome sequencing data for all isolates.
RESULTS
Genetic analysis identified four independent disruptions to the wciG gene, which encodes an O-acetyltransferase responsible for the O-acetylation of the 6Galβ1 residue in the capsular polysaccharide repeat unit of serotype 35B.
CONCLUSIONS
This is the first published report on the incidence and capsular gene characteristics of a S. pneumoniae 35B variant.
目的
从澳大利亚侵袭性肺炎球菌病病例中分离出的表现出非典型 35B 表型的肺炎链球菌分离株。使用全基因组测序分析这些菌株，并确定荚膜基因区域的变化。
方法
来自澳大利亚参考实验室的四个非典型血清群 35 分离株无法通过胶凝血清分型方法被分配到四个已知的血清群 35 型之一。通过对所有分离株的全基因组测序数据进行生物信息学分析，对荚膜基因座进行遗传特征分析。
结果
遗传分析确定了 wciG 基因的四个独立破坏，该基因编码负责荚膜多糖重复单元中 6Galβ1 残基 O-乙酰化的 O-乙酰基转移酶，血清型 35B。
结论
这是首次报道肺炎链球菌 35B 变体的发生率和荚膜基因特征。
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