Tamiello Chiara, Halder Maurice, Kamps Miriam A F, Baaijens Frank P T, Broers Jos L V, Bouten Carlijn V C
Department of Biomedical Engineering, Eindhoven University of Technology, P.O. Box 513, Eindhoven 5600 MB, The Netherlands.
Department of Molecular Cell Biology, CARIM School for Cardiovascular Diseases, Maastricht University, P.O. Box 616, Maastricht 6200 MD, The Netherlands.
J Cell Sci. 2017 Feb 15;130(4):779-790. doi: 10.1242/jcs.184838. Epub 2017 Jan 6.
In adherent cells, the relevance of a physical mechanotransduction pathway provided by the perinuclear actin cap stress fibers has recently emerged. Here, we investigate the impact of a functional actin cap on the cellular adaptive response to topographical cues and uniaxial cyclic strain. -deficient fibroblasts are used as a model system because they do not develop an intact actin cap, but predominantly form a basal layer of actin stress fibers underneath the nucleus. We observe that topographical cues induce alignment in both normal and -deficient fibroblasts, suggesting that the topographical signal transmission occurs independently of the integrity of the actin cap. By contrast, in response to cyclic uniaxial strain, -deficient cells show a compromised strain avoidance response, which is completely abolished when topographical cues and uniaxial strain are applied along the same direction. These findings point to the importance of an intact and functional actin cap in mediating cellular strain avoidance.
在贴壁细胞中，由核周肌动蛋白帽应力纤维提供的物理机械转导途径的相关性最近已显现出来。在这里，我们研究功能性肌动蛋白帽对细胞对地形线索和单轴循环应变的适应性反应的影响。缺乏[某种物质]的成纤维细胞被用作模型系统，因为它们不会形成完整的肌动蛋白帽，而是主要在细胞核下方形成一层肌动蛋白应力纤维的基底层。我们观察到，地形线索在正常和成纤维细胞缺乏的细胞中均诱导排列，这表明地形信号的传递独立于肌动蛋白帽的完整性而发生。相比之下，响应于循环单轴应变，缺乏[某种物质]的细胞表现出受损的应变规避反应，当沿着相同方向施加地形线索和单轴应变时，这种反应会完全消除。这些发现表明完整且功能性的肌动蛋白帽在介导细胞应变规避中的重要性。
