Mansour Lamjed, Azevedo Carlos, Alves Ângela, Al-Quraishy Saleh, Abdel-Baki Abdel-Azeem S
Zoology Department, College of Sciences, King Saud University, P.O. Box 2455, Riyadh, 11451, Saudi Arabia.
Unité de Recherche de Biologie intégrative et Ecologie évolutive et Fonctionnelle des Milieux Aquatiques, Département de Biologie, Faculté des Sciences de Tunis, Université De Tunis El Manar, Tunis, Tunisia.
Syst Parasitol. 2017 Jan;94(1):123-131. doi: 10.1007/s11230-016-9684-2. Epub 2017 Jan 6.
A new myxosporean parasite, Auerbachia maamouni n. sp., infecting the gallbladder of the golden trevally Gnathanodon speciosus Forsskål, is described, based on morphology, ultrastructure, and small subunit (SSU) rRNA gene sequencing. Of 50 fish collected from the Red Sea in Jeddah, Saudi Arabia, five were found heavily infected with mature myxospores floating free in the bile. Mature spores are pyriform to club-shaped with smooth valves, and contain a single polar capsule with an S-shaped polar filament, arranged in 13-16 polar filament coils, oriented longitudinally, with an irregular distribution on the polar capsule matrix. Spores measure 15.8 (14-17) μm in total length in lateral view, 7.9 (6-9) μm in width in apical view, with spore body length of 6.2 ± 0.4 (5-7) µm. The ellipsoidal polar capsule has two adjusted lateral folds 7.6 (6-8) µm long and 2 (2-3) µm wide. The new species is distinguished from other species of the genus based on spore morphometry and molecular data. The phylogenetic tree constructed using Maximum Likelihood and Bayesian Inference analysis of SSU rDNA sequence data supported the phylogenetic position of A. maamouni n. sp. among the species of Auerbachia Meglitsch, 1968 sequenced to date. Analysis of the SSU rDNA sequence data also supported the assumption that Auerbachia is closely related to members of the genera Coccomyxa Léger & Hesse, 1907, Zschokkella Auerbach, 1910 and Myxidium Bütschli, 1882, whose members inhabit the gallbladder of marine teleost fishes.
基于形态学、超微结构和小亚基（SSU）rRNA基因测序，描述了一种新的粘孢子虫寄生虫——马穆尼奥尔巴赫虫（Auerbachia maamouni n. sp.），它感染了金鲹（Gnathanodon speciosus Forsskål）的胆囊。在从沙特阿拉伯吉达红海采集的50条鱼中，发现有5条鱼被大量成熟的粘孢子严重感染，这些粘孢子游离在胆汁中。成熟孢子呈梨形至棒状，瓣膜光滑，含有一个单一的极囊，极丝呈S形，排列成13 - 16个极丝盘绕，纵向排列，在极囊基质上分布不规则。孢子在侧视图中的总长度为15.8（14 - 17）μm，在顶视图中的宽度为7.9（6 - 9）μm，孢子体长为6.2 ± 0.4（5 - 7）μm。椭圆形极囊有两条调整后的横向褶皱，长7.6（6 - 8）μm，宽2（2 - 3）μm。基于孢子形态测量和分子数据，该新物种与该属的其他物种相区分。使用最大似然法和贝叶斯推断分析SSU rDNA序列数据构建的系统发育树支持了马穆尼奥尔巴赫虫在迄今测序的1968年梅格利奇（Meglitsch）的奥尔巴赫虫属物种中的系统发育位置。对SSU rDNA序列数据的分析也支持了这样的假设，即奥尔巴赫虫与1907年莱热（Léger）和黑塞（Hesse）的球粘菌属（Coccomyxa）、1910年奥尔巴赫（Auerbach）的佐氏粘体虫属（Zschokkella）以及1882年比特施利（Bütschli）的粘孢子虫属（Myxidium）的成员密切相关，这些属的成员栖息在海洋硬骨鱼类的胆囊中。
