Ranjbar Hoda, Radahmadi Maryam, Reisi Parham, Alaei Hojjatallah
Department of Physiology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran.
Clin Exp Pharmacol Physiol. 2017 Apr;44(4):470-479. doi: 10.1111/1440-1681.12727.
Stress contributes, as a risk factor, to such psychological disorders as anxiety. The effects of electrical lesions in the basolateral amygdala nucleus (BLA) were investigated on the locomotor activity and anxiety-like behaviour in different stress durations. For this purpose, rats were randomly allocated to control, sham, and experimental groups, the latter including groups with and without BLA nucleus subjected to acute, sub-chronic, and chronic stress conditions for 1, 7, and 21 days, respectively, applied 6 h/d. The induced anxiety behaviour was evaluated using the open field test (OFT) while other variables were measured. Findings revealed that sub-chronic stress led to significantly reduced (P<.05) anxiety behaviours as measured by entries into and the time spent in the centre area while it also led to significant impairments in exploratory and locomotor activities, indicating intensified anxiety-like behaviour. BLA lesion affected rat behaviour differently such that it significantly (P<.05) decreased fear under sub-chronic and chronic stress conditions as evidenced by the subjects' greater tendency to enter the centre area in the open field test and their increased number of rearing events (P<.01). However, BLA lesion led to no significant decrease in the locomotor activity of subjects exposed to sub-chronic or chronic stress conditions as compared with those in similar groups but without BLA lesion. Finally, BLA lesion was found not only to decrease significantly (P<.01) adrenal gland and body weights, particularly under sub-chronic stress, but also to play a critical role in modulating adrenal functions by decreasing adrenal gland weight, and thereby reducing depression-like symptoms.
压力作为一个风险因素，会导致焦虑等心理障碍。研究了基底外侧杏仁核（BLA）电损伤对不同应激持续时间下大鼠运动活动和焦虑样行为的影响。为此，将大鼠随机分为对照组、假手术组和实验组，实验组包括有BLA核和无BLA核的组，分别在急性、亚慢性和慢性应激条件下持续1、7和21天，每天施加6小时。使用旷场试验（OFT）评估诱发的焦虑行为，同时测量其他变量。研究结果显示，亚慢性应激导致进入中央区域的次数和在中央区域停留的时间所衡量的焦虑行为显著减少（P<0.05），同时也导致探索和运动活动显著受损，表明焦虑样行为加剧。BLA损伤对大鼠行为的影响不同，在亚慢性和慢性应激条件下，大鼠进入中央区域的倾向增加以及站立次数增加（P<0.01），这表明BLA损伤显著（P<0.05）降低了恐惧。然而，与无BLA损伤的类似组相比，BLA损伤并未导致亚慢性或慢性应激条件下大鼠的运动活动显著下降。最后，发现BLA损伤不仅显著（P<0.01）降低肾上腺和体重，特别是在亚慢性应激下，而且通过降低肾上腺重量在调节肾上腺功能中起关键作用，从而减轻抑郁样症状。
